RAD21 and KIAA0196 at 8q24 are amplified and overexpressed in prostate cancer

To detect genes that are overexpressed in prostate cancer, a subtracted cDNA library was first constructed from the PC‐3 cell line and subsequently screened by using cDNA microarray hybridization. Sixty‐eight genes were found to be overexpressed (ratio > 3) in PC‐3. Half of these genes were in ch...

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Bibliographic Details
Published in:Genes chromosomes & cancer 2004-01, Vol.39 (1), p.1-10
Main Authors: Porkka, Kati P., Tammela, Teuvo L.J., Vessella, Robert L., Visakorpi, Tapio
Format: Article
Language:English
Subjects:
Blotting, Northern
Cell Line, Tumor
chromosome 8
Chromosomes, Human, Pair 8 - genetics
DNA microarrays
DNA, Complementary - genetics
Gene Amplification
Gene Dosage
Gene Expression Profiling
Gene Expression Regulation, Neoplastic - genetics
Genes, Neoplasm - genetics
Humans
In Situ Hybridization, Fluorescence
KIAA0196 gene
Male
Nuclear Proteins - genetics
Nucleic Acid Hybridization
Oligonucleotide Array Sequence Analysis
Phosphoproteins - genetics
Prostatic Neoplasms - genetics
Prostatic Neoplasms - metabolism
Prostatic Neoplasms - pathology
RAD21 gene
Reverse Transcriptase Polymerase Chain Reaction
Transplantation, Heterologous
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Summary:To detect genes that are overexpressed in prostate cancer, a subtracted cDNA library was first constructed from the PC‐3 cell line and subsequently screened by using cDNA microarray hybridization. Sixty‐eight genes were found to be overexpressed (ratio > 3) in PC‐3. Half of these genes were in chromosomal regions, which, using comparative genomic hybridization, we previously showed to be gained in PC‐3. Subsequently, the expression and copy number of selected genes were studied by quantitative RT‐PCR and fluorescence in situ hybridization in prostate cancer cell lines, xenografts, and clinical tumor specimens of benign prostate hyperplasia and untreated as well as hormone‐refractory prostate carcinomas. Two genes from chromosomal region 8q24—RAD21 and KIAA0196—showed increased expression in clinical prostate carcinomas and were also amplified in 30–40% of xenografts and hormone‐refractory tumors. In addition, the expression of KIAA0196 was significantly (P = 0.0051) higher in tumors with the gene amplification than in those without it. The data suggest that KIAA0196 and possibly RAD21 are putative target genes for the common amplification of 8q23‐24 in prostate cancer. © 2003 Wiley‐Liss, Inc.
ISSN:1045-2257
1098-2264
DOI:10.1002/gcc.10289