Immunohistochemical demonstration of Francisella tularensis in lesions of cats with tularemia

Department of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan 66506, USA. An immunohistochemical test was developed and validated for detection of Francisella tularensis antigen in tissues of cats with fatal tularemia. Ten cases of naturally occurring tularemia in cats were posi...

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Bibliographic Details
Published in:Journal of veterinary diagnostic investigation 2002-03, Vol.14 (2), p.162-164
Main Authors: DeBey, BM, Andrews, GA, Chard-Bergstrom, C, Cox, L
Format: Article
Language:English
Subjects:
Animals
Antigens, Bacterial - analysis
Biological and medical sciences
Cat Diseases - diagnosis
Cat Diseases - immunology
Cats
Francisella tularensis - immunology
Francisella tularensis - isolation & purification
Francisella tularensis - pathogenicity
Fundamental and applied biological sciences. Psychology
Immunohistochemistry - veterinary
Microbiology
Tularemia - diagnosis
Tularemia - immunology
Tularemia - veterinary
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Summary:Department of Diagnostic Medicine/Pathobiology, Kansas State University, Manhattan 66506, USA. An immunohistochemical test was developed and validated for detection of Francisella tularensis antigen in tissues of cats with fatal tularemia. Ten cases of naturally occurring tularemia in cats were positive both by isolation of F. tularensis and immunohistochemical identification of F. tularensis antigen. Nine additional cases with lesions typical of tularemia were positive for F. tularensis antigen, although bacterial cultures were not performed. Immunohistochemical identification of F. tularensis in formalin-fixed tissue is valuable for establishing a rapid etiologic diagnosis under circumstances where fresh tissues may not be available for isolation and identification of the organism.
ISSN:1040-6387
1943-4936
DOI:10.1177/104063870201400213