Discrepancies in the immunophenotype of lymphoma cells in samples obtained simultaneously from different anatomic sites

Few studies have compared the immunophenotypic profiles of non-Hodgkin lymphoma (NHL) cells obtained simultaneously from different anatomic sites. In the present study, we compared flow cytometry immunophenotypic results in 64 consecutive NHL cases in which aspiration or biopsy of 2 sites was perfor...

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Bibliographic Details
Published in:American journal of clinical pathology 2002-04, Vol.117 (4), p.644-650
Main Authors: ONCIU, Mihaela, BERRAK, Su G, MEDEIROS, L. Jeffrey, KATZ, Ruth L, HUH, Yang O
Format: Article
Language:English
Subjects:
Adult
Aged
Antigens, CD20 - analysis
Biological and medical sciences
Biopsy
Biopsy, Needle
Bone Marrow - immunology
Bone Marrow - pathology
CD5 Antigens - analysis
Female
Flow Cytometry
Glycoproteins - analysis
Hematologic and hematopoietic diseases
Humans
Immunophenotyping
Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis
Lymph Nodes - immunology
Lymph Nodes - pathology
Lymphoma, Non-Hodgkin - drug therapy
Lymphoma, Non-Hodgkin - immunology
Lymphoma, Non-Hodgkin - pathology
Male
Medical sciences
Middle Aged
Receptors, IgE - analysis
Retrospective Studies
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Summary:Few studies have compared the immunophenotypic profiles of non-Hodgkin lymphoma (NHL) cells obtained simultaneously from different anatomic sites. In the present study, we compared flow cytometry immunophenotypic results in 64 consecutive NHL cases in which aspiration or biopsy of 2 sites was performed within 30 days to assess the potential discrepancy rate. In 14 cases (22%), discordant antigen expression was identified, including 4 (36%) of 11 cases with discordant morphologic features and 10 (19%) of 53 cases with concordant morphologic features in the 2 samples. Discrepancies involved 1 antigen in 10 patients and 2 antigens in 4 patients. Antigens most frequently discrepant included CD5 (n = 4), FMC7 (n = 3), and CD20 (n = 3). We conclude that the immunophenotype of NHL cells is generally stable, yet discrepancies can occur in a subset of patients. Differences in immunophenotype may relate to mechanisms of disease dissemination, influence of the microenvironment, or differential response to therapy.
ISSN:0002-9173
1943-7722
DOI:10.1309/URTD-7MD9-U8N1-C60Q