A novel HLA-A allele: A0257

:  A novel human leucocyte antigen‐A*02 (HLA‐A*02) allele was detected by reference strand‐mediated conformation analysis (RSCA) of a DNA sample from a Tarahumara individual. Direct sequencing of HLA‐A locus polymerase chain reaction products identified a mutation in one of the alleles. Cloning and...

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Bibliographic Details
Published in:Tissue antigens 2004-01, Vol.63 (1), p.85-87
Main Authors: García-Ortiz, J.E., Cox, S.T., Sandoval-Ramirez, L., Little, A.M., Marsh, S.G.E., Madrigal, J.A., Argüello, J.R.
Format: Article
Language:English
Subjects:
Alleles
Base Sequence
HLA-A Antigens - genetics
HLA-A Antigens - immunology
HLA-A0257
Humans
Molecular Sequence Data
Mutation
new HLA allele
Polymerase Chain Reaction
reference strand-mediated conformation analysis
Sequence Analysis, DNA
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Summary::  A novel human leucocyte antigen‐A*02 (HLA‐A*02) allele was detected by reference strand‐mediated conformation analysis (RSCA) of a DNA sample from a Tarahumara individual. Direct sequencing of HLA‐A locus polymerase chain reaction products identified a mutation in one of the alleles. Cloning and sequencing confirmed the presence of a new allele, A*0257 which differed from A*0206 by two nucleotides at positions 355 and 362, inducing changes in residues 95 and 97, respectively, within the peptide‐binding site. Those changes suggest that allele A*0257 may have resulted from an intralocus recombination event.
ISSN:0001-2815
1399-0039
DOI:10.1111/j.1399-0039.2004.00154.x