Fibronectin peptides mediate HMEC adhesion to porcine-derived extracellular matrix

Extracellular matrices (ECM) derived from porcine tissues have been shown to support the successful repair and remodeling of injured tissues when evaluated in animal models. Cell–matrix interactions, including ligand–integrin associations that facilitate endothelial cell adhesion, are clearly import...

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Bibliographic Details
Published in:Biomaterials 2002-04, Vol.23 (8), p.1841-1848
Main Authors: Hodde, Jason, Record, Rae, Tullius, Robert, Badylak, Stephen
Format: Article
Language:English
Subjects:
Adhesion
Animals
Cations
Cattle
Cell Adhesion
Endothelial cell
Endothelium, Vascular - cytology
Extracellular matrix
Extracellular Matrix - metabolism
Fibronectin
Fibronectins - metabolism
Fibronectins - pharmacology
Humans
Integrins
Integrins - metabolism
Intestinal Mucosa - pathology
Ligands
Microcirculation - cytology
Peptides - chemistry
Peptides - pharmacology
Protein Binding
Small intestinal submucosa
Swine
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Summary:Extracellular matrices (ECM) derived from porcine tissues have been shown to support the successful repair and remodeling of injured tissues when evaluated in animal models. Cell–matrix interactions, including ligand–integrin associations that facilitate endothelial cell adhesion, are clearly important in the tissue remodeling process. The goal of the present study was to identify the peptide sequences within the ubiquitous protein fibronectin (FN) that may be important in the initial interactions between the host endothelial cells and the ECM scaffold. Human microvascular endothelial cells (HMEC) were seeded upon porcine ECM after having been subjected to pretreatment with peptide ligands derived from tissue FN and were allowed to attach for 20min. Non-adherent cells were removed and the remaining, tritium-labeled cells attached to the ECM were counted. Results showed that cyclo-RGD and REDV, but not LDV or PHSRN, play a role in mediating the attachment of HMEC to porcine ECM.
ISSN:0142-9612
1878-5905
DOI:10.1016/S0142-9612(01)00310-6