A novel reporter strain to follow Cre-mediated recombination in T and NK cells

The Cre‐loxP system permits the generation of mouse models in which the fate of a cell can be followed through time. Such approach is of great value in immunology because it may allow lineage studies and the dissection of the contribution of specific effector T cells to long‐term memory responses or...

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Bibliographic Details
Published in:Genesis (New York, N.Y. : 2000) N.Y. : 2000), 2002-04, Vol.32 (4), p.287-292
Main Authors: Wurtz, Olivier, Pophillat, Matthieu, Schmitt-Verhulst, Anne-Marie, Guerder, Sylvie
Format: Article
Language:English
Subjects:
Animals
CD2 Antigens - genetics
Cre
Genes, Reporter
Integrases - genetics
Integrases - metabolism
Killer Cells, Natural - enzymology
Lymph Nodes - immunology
Mice
molecular biology
Recombination, Genetic
Restriction Mapping
Sequence Deletion
T-Lymphocytes - enzymology
transgenic mice
Viral Proteins - genetics
Viral Proteins - metabolism
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Summary:The Cre‐loxP system permits the generation of mouse models in which the fate of a cell can be followed through time. Such approach is of great value in immunology because it may allow lineage studies and the dissection of the contribution of specific effector T cells to long‐term memory responses or autoimmune responses. An essential component of such a strategy is the development of appropriate reporter strains of mice in which the inducible reporter molecule is not immunogenic and is well expressed at the cell surface of T cells. We describe here a novel reporter strain of mice that is designed to fulfill these criteria and show that this strain permits the monitoring of Cre‐mediated recombination in both T cells and NK cells. genesis 32:287–292, 2002. © 2002 Wiley‐Liss, Inc.
ISSN:1526-954X
1526-968X
DOI:10.1002/gene.10082