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Draw-fill batch culture mode for production of xylanases by Cellulomonas flavigena on sugar cane bagasse

Draw-fill culture was evaluated as a method for xylanase production by Cellulomonas flavigena on sugar cane bagasse. Specific xylanase activity and volumetric xylanase activities were measured by harvesting 50%, 55%, 60% and 70% of fermented broth at the end of each subculture. Maximum specific (64...

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Bibliographic Details
Published in:Applied microbiology and biotechnology 2002-03, Vol.58 (4), p.435-438
Main Authors: Vega-Estrada, J, Flores-Cotera, L.B, Santiago, A, Magana-Plaza, I, Montes-Horcasitas, C
Format: Article
Language:English
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Summary:Draw-fill culture was evaluated as a method for xylanase production by Cellulomonas flavigena on sugar cane bagasse. Specific xylanase activity and volumetric xylanase activities were measured by harvesting 50%, 55%, 60% and 70% of fermented broth at the end of each subculture. Maximum specific (64 IU mg(-1) protein) and volumetric (166 IU ml(-1)) xylanase activities were obtained by harvesting 50-55% of broth. Values were 3.4 and 3.8 times greater than those obtained in batch cultures carried out under the same conditions. Enzyme productivity of 4.2 IU ml(-1) h(-1) was significantly greater than that obtained in continuous cultures (2.4 IU ml(-1) h(-1)) (P
ISSN:0175-7598
1432-0614
DOI:10.1007/s00253-001-0908-1