Base-Dependent Competitive Adsorption of Single-Stranded DNA on Gold

We characterize the room-temperature adsorption of single-stranded DNA homo-oligonucleotides from solution onto polycrystalline Au films, including competitive adsorption between all possible pairs of unmodified oligomers. Fourier transform infrared (FTIR) and X-ray photoelectron (XPS) spectroscopy...

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Bibliographic Details
Published in:Journal of the American Chemical Society 2003-07, Vol.125 (30), p.9014-9015
Main Authors: Kimura-Suda, Hiromi, Petrovykh, Dmitri Y, Tarlov, Michael J, Whitman, Lloyd J
Format: Article
Language:English
Subjects:
Adsorption
Base Pairing
Binding, Competitive
Biological and medical sciences
DNA, Single-Stranded - chemistry
Fundamental and applied biological sciences. Psychology
Gold - chemistry
Molecular biophysics
Spectroscopy, Fourier Transform Infrared
Surface properties. Adsorption
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Summary:We characterize the room-temperature adsorption of single-stranded DNA homo-oligonucleotides from solution onto polycrystalline Au films, including competitive adsorption between all possible pairs of unmodified oligomers. Fourier transform infrared (FTIR) and X-ray photoelectron (XPS) spectroscopy analysis of the resulting films shows that oligonucleotides adsorb with a strongly base-dependent affinity, adenine (A) > cytosine (C) ≥ guanine (G) > thymine (T). In competitive adsorption experiments on Au, oligo(dA) strongly dominates over the other oligonucleotides. The relative adsorption affinity of oligo(dA) is so great that it competes effectively against adsorption of thiolated oligomers and even causes hybridized oligo(dA)·oligo(dT) duplexes to denature in the presence of Au.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja035756n