Base-Dependent Competitive Adsorption of Single-Stranded DNA on Gold

We characterize the room-temperature adsorption of single-stranded DNA homo-oligonucleotides from solution onto polycrystalline Au films, including competitive adsorption between all possible pairs of unmodified oligomers. Fourier transform infrared (FTIR) and X-ray photoelectron (XPS) spectroscopy...

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Published in:Journal of the American Chemical Society 2003-07, Vol.125 (30), p.9014-9015
Main Authors: Kimura-Suda, Hiromi, Petrovykh, Dmitri Y, Tarlov, Michael J, Whitman, Lloyd J
Format: Article
Language:English
Subjects:
Adsorption
Base Pairing
Binding, Competitive
Biological and medical sciences
DNA, Single-Stranded - chemistry
Fundamental and applied biological sciences. Psychology
Gold - chemistry
Molecular biophysics
Spectroscopy, Fourier Transform Infrared
Surface properties. Adsorption
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cited_by cdi_FETCH-LOGICAL-a379t-825210ae62fa43c571c8e13ae36e60f6011e037b3e6c323ba45c9f4205014cbe3
cites cdi_FETCH-LOGICAL-a379t-825210ae62fa43c571c8e13ae36e60f6011e037b3e6c323ba45c9f4205014cbe3
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creator Kimura-Suda, Hiromi
Petrovykh, Dmitri Y
Tarlov, Michael J
Whitman, Lloyd J
description We characterize the room-temperature adsorption of single-stranded DNA homo-oligonucleotides from solution onto polycrystalline Au films, including competitive adsorption between all possible pairs of unmodified oligomers. Fourier transform infrared (FTIR) and X-ray photoelectron (XPS) spectroscopy analysis of the resulting films shows that oligonucleotides adsorb with a strongly base-dependent affinity, adenine (A) > cytosine (C) ≥ guanine (G) > thymine (T). In competitive adsorption experiments on Au, oligo(dA) strongly dominates over the other oligonucleotides. The relative adsorption affinity of oligo(dA) is so great that it competes effectively against adsorption of thiolated oligomers and even causes hybridized oligo(dA)·oligo(dT) duplexes to denature in the presence of Au.
doi_str_mv 10.1021/ja035756n
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source American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list)
subjects Adsorption
Base Pairing
Binding, Competitive
Biological and medical sciences
DNA, Single-Stranded - chemistry
Fundamental and applied biological sciences. Psychology
Gold - chemistry
Molecular biophysics
Spectroscopy, Fourier Transform Infrared
Surface properties. Adsorption
title Base-Dependent Competitive Adsorption of Single-Stranded DNA on Gold
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