Diminished Th1 and Th2 Cytokine Production in Healthy Adult Nonresponders to Recombinant Hepatitis B Vaccine

Vaccination of healthy adults with recombinant hepatitis B (rHB) vaccine fails to induce a protective antibody response in a proportion of individuals. Imbalanced T‐helper (Th)1/Th2 response has been attributed to the lack of specific antibody response to rHB vaccine. In this study, in vitro product...

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Bibliographic Details
Published in:Scandinavian journal of immunology 2002-03, Vol.55 (3), p.311-314
Main Authors: Kardar, G. A., Jeddi‐Tehrani, M., Shokri, F.
Format: Article
Language:English
Subjects:
Adult
Cytokines - biosynthesis
Female
g-Interferon
Hepatitis B
Hepatitis B Surface Antigens - administration & dosage
Hepatitis B Vaccines - pharmacology
Hepatitis B virus
Humans
Immune Tolerance
In Vitro Techniques
Interferon-gamma - biosynthesis
Interleukin-10 - biosynthesis
Interleukin-2 - biosynthesis
Lymphocyte Activation
Male
Phytohemagglutinins - administration & dosage
Th1 Cells - immunology
Th2 Cells - immunology
Vaccines, DNA - pharmacology
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Summary:Vaccination of healthy adults with recombinant hepatitis B (rHB) vaccine fails to induce a protective antibody response in a proportion of individuals. Imbalanced T‐helper (Th)1/Th2 response has been attributed to the lack of specific antibody response to rHB vaccine. In this study, in vitro production of interleukin (IL)‐2, interferon (IFN)‐γ and IL‐10 was investigated in Iranian healthy adults vaccinated with rHB vaccine. Peripheral blood mononuclear cells (PBMC) were isolated from 18 high responders and eight nonresponders and stimulated with rHB antigen or phytohaemaglutinin (PHA) mitogen. The cytokines were quantitated in culture supernatants by sandwich enzyme‐linked immunosorbent assay (ELISA). Our results demonstrated a significant decrease in the production of IL‐2, IFN‐γ and IL‐10 (P 
ISSN:0300-9475
1365-3083
DOI:10.1046/j.1365-3083.2002.01057.x