Fragmentation of Sialylated Carbohydrates Using Infrared Atmospheric Pressure MALDI Ion Trap Mass Spectrometry from Cation-Doped Liquid Matrixes

Infrared atmospheric pressure matrix-assisted laser desorption/ionization on an ion trap mass spectrometer is used to study sialylated oligosaccharides desorbed from the liquid phase. Glycerol doped with various cations provides the opportunity to produce cation-adducted intact molecular ions of sug...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2003-12, Vol.75 (23), p.6523-6530
Main Authors: Von Seggern, Christopher E, Zarek, Paul E, Cotter, Robert J
Format: Article
Language:English
Subjects:
Analytical chemistry
Atmospheric Pressure
Carbohydrate Conformation
Carbohydrate Sequence
Carbohydrates
Cations
Chemistry
Cobalt
Exact sciences and technology
Infrared imaging systems
Infrared Rays
Molecular Sequence Data
N-Acetylneuraminic Acid - chemistry
Oligosaccharides - chemistry
Pressure
Salts
Scientific imaging
Spectrometric and optical methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods
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Summary:Infrared atmospheric pressure matrix-assisted laser desorption/ionization on an ion trap mass spectrometer is used to study sialylated oligosaccharides desorbed from the liquid phase. Glycerol doped with various cations provides the opportunity to produce cation-adducted intact molecular ions of sugars. Distinct combinations of cations allow for sialic acid stabilization, as well as differential cleavage, resulting in more complete fragmentation coverage of the oligosaccharide. Alkali and transition metal cations are utilized to create three distinct molecular ion species, involving the adduction of a singly charged cation, two singly charged cations, or a doubly charged cation. From these different molecular ion types, complementary sequence, branching, and linkage information for sialylated oligosaccharides can be deduced.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac0348367