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Induction of apoptosis in vascular smooth muscle cells by mechanical stretch

1  Department of Bioengineering and Whitaker Institute of Biomedical Engineering, 2  Advanced Tissue Sciences, La Jolla 92037; 3  Division of Biomedical Sciences, University of California, Riverside 92506; and 4  Department of Medicine, University of California, San Diego, La Jolla, California 92093...

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Published in:American journal of physiology. Heart and circulatory physiology 2002-05, Vol.282 (5), p.H1709-H1716
Main Authors: Sotoudeh, Mohammad, Li, Yi-Shuan, Yajima, Noriyuki, Chang, Chih-Chieh, Tsou, Tsui-Chun, Wang, Yibin, Usami, Shunichi, Ratcliffe, Anthony, Chien, Shu, Shyy, John Y.-J
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Language:English
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Summary:1  Department of Bioengineering and Whitaker Institute of Biomedical Engineering, 2  Advanced Tissue Sciences, La Jolla 92037; 3  Division of Biomedical Sciences, University of California, Riverside 92506; and 4  Department of Medicine, University of California, San Diego, La Jolla, California 92093 We studied the response of porcine vascular smooth muscle cells (PVSMCs) to cyclic sinusoidal stretch at a frequency of 1   Hz. Cyclic stretch with an area change of 25% caused an increase in PVSMC apoptosis, which was accompanied by sustained activation of c-Jun NH 2 -terminal kinases (JNK) and the mitogen-activated protein kinase p38. Cyclic stretch with an area change of 7% had no such effect. Infection of PVSMCs with recombinant adenoviruses expressing constitutively active forms of upstream molecules that activate JNK and p38 also led to apoptosis. The simultaneous blockade of both JNK and p38 pathways with adenovirus-mediated expression of dominant-negative mutants of c-Jun and p38 caused a significant decrease (to 1/2) of the apoptosis induced by 25% cyclic stretch. The 25% stretch also caused sustained clustering of tumor necrosis factor- (TNF- ) receptor-1 and its association with TNF- receptor-associated factor-2 (TRAF-2). Overexpressing the wild-type TRAF-2 in PVSMCs caused an increase in apoptosis. In contrast, the expression of a dominant-negative mutant of TRAF-2 attenuated stretch-induced apoptois. These results support the hypothesis that circumferential overload under hypertensive conditions induces a clustering of death receptors that cause vascular smooth muscle cell apoptosis. c-Jun NH 2 -terminal kinases; p38; mechanotransduction; mechanical overload; vascular wall
ISSN:0363-6135
1522-1539
DOI:10.1152/ajpheart.00744.2001