Diabetes-related changes in rat cerebral occludin and zonula occludens-1 (ZO-1) expression

The endothelial or epithelial tight junctions create a barrier to diffusion of solutes. Since experimental diabetes mellitus is associated with considerable alterations in the blood-brain barrier (BBB), it is possible that specific tight junction proteins may be altered in diabetes. To test this hyp...

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Bibliographic Details
Published in:Neurochemical research 2002-03, Vol.27 (3), p.249-252
Main Authors: CHEHADE, Joe M, HAAS, Michael J, MOORADIAN, Arshag D
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Brain - metabolism
Diabetes Mellitus, Experimental - genetics
Diabetes. Impaired glucose tolerance
Endocrine pancreas. Apud cells (diseases)
Endocrinopathies
Etiopathogenesis. Screening. Investigations. Target tissue resistance
Gene Expression Regulation
Insulin - pharmacology
Male
Medical sciences
Membrane Proteins - genetics
Occludin
Phosphoproteins - genetics
Rats
Rats, Inbred F344
Reference Values
Transcription, Genetic
Zonula Occludens-1 Protein
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Summary:The endothelial or epithelial tight junctions create a barrier to diffusion of solutes. Since experimental diabetes mellitus is associated with considerable alterations in the blood-brain barrier (BBB), it is possible that specific tight junction proteins may be altered in diabetes. To test this hypothesis, Western and Northern blot analysis were carried out to measure the steady-state level of occludin and zonula occludens-one (ZO-1) proteins and mRNA levels in cerebral tissue of streptozotocin-induced diabetic rats and the results were compared to insulin treated diabetic rats and vehicle injected control rats. The cerebral occludin content in diabetic rats (115.4 +/- 18.6 arbitrary units) was significantly reduced compared to insulin-treated diabetic rats (649.1 +/- 141.2) or control rats (552.9 +/- 82.9), p < 0.001. The ZO-1 content of cerebral tissue from diabetic rats (1,240.6 +/- 199.7 arbitrary units) was not significantly altered compared to controls (1,310.8 +/- 256.9). The cerebral occludin mRNA content relative to G3PDH mRNA was 1.35 +/- 0.07 and 1.34 +/- 0.19 in control and diabetic rats respectively. The cerebral ZO-1 mRNA content relative to G3PDH mRNA in diabetic and control rats was 1.135 +/- 0.123 and 0.956 +/- 0.038 respectively. These differences did not achieve statistical significance. It is concluded that diabetes alters the molecular anatomy of the tight junctions in cerebral tissue by altering the content of select structural proteins.
ISSN:0364-3190
1573-6903
DOI:10.1023/A:1014892706696