Tat protein expression in MDBK cells does not confer susceptibility to bovine immunodeficiency virus

The ability of BIV strain R29 to infect bovine cell lines in the presence or absence of a functional lentiviral Tat protein is described. Jembrana disease virus (JDV) Tat protein was stably expressed in MDBK cells. No viral replication could be detected in this cell line after infection with BIV R29...

Full description

Saved in:
Bibliographic Details
Published in:Archives of virology 2002-03, Vol.147 (3), p.643-649
Main Authors: KEMPSTER, S, COLLINS, M. E, BROWNLIE, J
Format: Article
Language:English
Subjects:
Animal viral diseases
Animals
Biological and medical sciences
Bovine immunodeficiency syndrome
Bovine immunodeficiency virus
Cattle
Cell culture
Cell Line
Cloning
DNA, Viral - physiology
Enzymes
Epidemiology
Fundamental and applied biological sciences. Psychology
Gene Products, tat - genetics
Gene Products, tat - metabolism
Genomes
Immunodeficiency Virus, Bovine - genetics
Immunodeficiency Virus, Bovine - pathogenicity
Immunodeficiency Virus, Bovine - physiology
Infections
Infectious diseases
Jembrana disease virus
Lentiviruses, Bovine - genetics
Lentiviruses, Bovine - metabolism
Medical sciences
Microbiology
Protein expression
Proteins
Proviruses
Transfection
Viral diseases
Virology
Virus Replication
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The ability of BIV strain R29 to infect bovine cell lines in the presence or absence of a functional lentiviral Tat protein is described. Jembrana disease virus (JDV) Tat protein was stably expressed in MDBK cells. No viral replication could be detected in this cell line after infection with BIV R29. Transfection of MDBK cells and MDBK Tat expressing cells with BIV R29 proviral DNA established that BIV R29 could not replicate in MDBK cells. Whether viral entry into MDBK cells is also a block to BIV R29 infection of MDBK cells has yet to be established.
ISSN:0304-8608
1432-8798
DOI:10.1007/s007050200014