Widespread Translational Inhibition by Plant miRNAs and siRNAs

High complementarity between plant microRNAs (miRNAs) and their messenger RNA targets is thought to cause silencing, prevalently by endonucleolytic cleavage. We have isolated Arabidopsis mutants defective in miRNA action. Their analysis provides evidence that plant miRNA-guided silencing has a wides...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 2008-05, Vol.320 (5880), p.1185-1190
Main Authors: Brodersen, Peter, Sakvarelidze-Achard, Lali, Bruun-Rasmussen, Marianne, Dunoyer, Patrice, Yamamoto, Yoshiharu Y, Sieburth, Leslie, Voinnet, Olivier
Format: Article
Language:English
Subjects:
Adenosine Triphosphatases - genetics
Adenosine Triphosphatases - physiology
Arabidopsis
Arabidopsis - genetics
Arabidopsis - physiology
Arabidopsis Proteins - genetics
Arabidopsis Proteins - physiology
Argonaute Proteins
Biological and medical sciences
Botany
Enzymes
Fundamental and applied biological sciences. Psychology
Gene expression regulation
Gene Expression Regulation, Plant
Green Fluorescent Proteins - genetics
Katanin
Messenger RNA
MicroRNA
MicroRNAs - physiology
Molecular and cellular biology
Molecular biology
Molecular genetics
Mutation
Phenotypes
Plant cells
Protein Biosynthesis
Repression
Reverse transcriptase polymerase chain reaction
Ribonucleic acid
RNA
RNA Caps
RNA Interference
RNA, Plant - physiology
RNA, Small Interfering - physiology
Seedlings
Small interfering RNA
Translation. Translation factors. Protein processing
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Summary:High complementarity between plant microRNAs (miRNAs) and their messenger RNA targets is thought to cause silencing, prevalently by endonucleolytic cleavage. We have isolated Arabidopsis mutants defective in miRNA action. Their analysis provides evidence that plant miRNA-guided silencing has a widespread translational inhibitory component that is genetically separable from endonucleolytic cleavage. We further show that the same is true of silencing mediated by small interfering RNA (siRNA) populations. Translational repression is effected in part by the ARGONAUTE proteins AGO1 and AGO10. It also requires the activity of the microtubule-severing enzyme katanin, implicating cytoskeleton dynamics in miRNA action, as recently suggested from animal studies. Also as in animals, the decapping component VARICOSE (VCS)/Ge-1 is required for translational repression by miRNAs, which suggests that the underlying mechanisms in the two kingdoms are related.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.1159151