Serological and coat protein sequence studies suggest that necrosis disease on sunflower in India is caused by a strain of Tobacco streak ilarvirus

Serological and coat protein sequence studies were conducted to identify an ilarvirus associated with necrosis disease on sunflower in India. In electroblot immunoassay, sunflower ilarvirus reacted strongly only with antiserum to Tobacco streak virus (TSV). The coat protein gene of sunflower ilarvir...

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Bibliographic Details
Published in:Archives of virology 2002-03, Vol.147 (3), p.651-658
Main Authors: BHAT, A. I, JAIN, R. K, KUMAR, A, RAMIAH, M, VARMA, A
Format: Article
Language:English
Subjects:
Antibodies, Viral - immunology
Automation
Bacteriology
Base Sequence
Biological and medical sciences
Capsid - genetics
Capsid - immunology
Cloning
Fundamental and applied biological sciences. Psychology
Genetics
Genomes
Helianthus - virology
Helianthus annuus
Human mycoses
Ilarvirus - classification
Ilarvirus - genetics
Ilarvirus - immunology
Ilarvirus - isolation & purification
Immunoassay
India
Infectious diseases
Medical sciences
Microbiology
Molecular Sequence Data
Mycoses
Mycoses of the skin
Plant Diseases - virology
Plant pathology
Proteins
Sequence Analysis, DNA
Serology
Tobacco
Tobacco streak virus
Viruses
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Summary:Serological and coat protein sequence studies were conducted to identify an ilarvirus associated with necrosis disease on sunflower in India. In electroblot immunoassay, sunflower ilarvirus reacted strongly only with antiserum to Tobacco streak virus (TSV). The coat protein gene of sunflower ilarvirus was cloned and sequenced. The sequence analyses also showed that the CP gene was most closely related to TSV, the member of subgroup I of Ilarvirus. The sunflower ilarvirus CP shared 90% amino acid sequence identity with TSV. On the basis of serological relatedness and sequence identity, it is proposed that the sunflower ilarvirus from India should be considered a strain of TSV belonging to subgroup I and designated as TSV-SF. This is the first report of the molecular characterization of TSV on sunflower from the Indian subcontinent.
ISSN:0304-8608
1432-8798
DOI:10.1007/s007050200015