Antihyperglycemic and antilipidperoxidative effects of dry matter of culture broth of Inonotus obliquus in submerged culture on normal and alloxan-diabetes mice

• Published in: Journal of ethnopharmacology 2008-06, Vol.118 (1), p.7-13
• Main Authors: Sun, Jun-En, Ao, Zong-Hua, Lu, Zhen-Ming, Xu, Hong-Yu, Zhang, Xiao-Mei, Dou, Wen-Fang, Xu, Zheng-Hong
• Format: Article
• Language: English
• Subjects: Alloxan; animal models; Animals; Antihyperglycemic; Antilipidperoxidative; Antioxidant; antioxidant activity; Antioxidants - administration & dosage; Antioxidants - isolation & purification; Antioxidants - pharmacology; Basidiomycota - chemistry; Biological and medical sciences; blood glucose; Blood Glucose - drug effects; blood lipids; catalase; Culture Media; Diabetes; diabetes mellitus; Diabetes Mellitus, Experimental - drug therapy; dosage; General pharmacology; glibenclamide; glutathione peroxidase; Glyburide - pharmacology; glycemic control; glycemic effect; histopathology; Hypoglycemic Agents - administration & dosage; Hypoglycemic Agents - isolation & purification; Hypoglycemic Agents - pharmacology; Inonotus; Inonotus obliquus; insulin; lipid peroxidation; Lipid Peroxidation - drug effects; Lipids - blood; Male; Medical sciences; medicinal properties; Mice; Mice, Inbred ICR; pancreas; Pancreas - drug effects; Pancreas - pathology; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; superoxide dismutase; tissue culture
• ISSN: 0378-8741; 1872-7573
• DOI: 10.1016/j.jep.2008.02.030

The antihyperglycemic and antilipidperoxidative effects of the dry matter of culture broth (DMCB) of Inonotus obliquus were investigated. The normal, glucose-induced hyperglycemic and alloxan-induced diabetic mice were used to evaluate the antihyperglycemic and antilipidperoxidative effects of the DMCB of Inonotus obliquus. Treatment with the DMCB (500 and 1000 mg/kg body weight) exhibited a mild hypoglycemic effect in normal mice, and failed to reduce the peak glucose levels after glucose administration. However, euglycemia was achieved in the DMCB of Inonotus obliquus (1000 mg/kg) and glibenclamide-treated mice after 120 min of glucose loading. In alloxan-induced diabetic mice, the DMCB (500 and 1000 mg/kg body weight for 21 days) showed a significant decrease in blood glucose level, the percentages reduction on the 7th day were 11.90 and 15.79%, respectively. However, feeding of this drug for 3 weeks produced reduction was 30.07 and 31.30%. Furthermore, the DMCB treatment significantly decreased serum contents of free fatty acid (FFA), total cholesterol (TC), triglyceride (TG) and low density lipoprotein-cholesterol (LDL-C), whereas effectively increased high density lipoprotein-cholesterol (HDL-C), insulin level and hepatic glycogen contents in liver on diabetic mice. Besides, the DMCB treatment significantly increased catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) activities except for decreasing maleic dialdehyde (MDA) level in diabetic mice. Histological morphology examination showed that the DMCB restored the damage of pancreas tissues in mice with diabetes mellitus. The results showed that the DMCB of Inonotus obliquus possesses significant antihyperglycemic, antilipidperoxidative and antioxidant effects in alloxan-induced diabetic mice.