Characterization of two CTR‐like protein kinases in Rosa hybrida and their expression during flower senescence and in response to ethylene

• Published in: Journal of experimental botany 2002-05, Vol.53 (371), p.1223-1225
• Main Authors: Müller, Renate, Owen, Carolyn A., Xue, Zhong‐Tian, Welander, Margareta, Stummann, Bjarne M.
• Format: Article
• Language: English
• Subjects: Agroecology; Amino Acid Sequence; Amino acids; Biological and medical sciences; Cellular senescence; Complementary DNA; CTR‐homologues; DNA, Complementary - chemistry; DNA, Complementary - genetics; Ecological genetics; Enzymes; ethylene; Ethylenes - pharmacology; flower senescence; Flowers; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Enzymologic - drug effects; Gene Expression Regulation, Plant - drug effects; GENE NOTE; Metabolism; Molecular Sequence Data; Petals; Phylogeny; Plant Growth Regulators - pharmacology; Plant physiology and development; Plant Proteins - genetics; Plant Stems - enzymology; Plant Stems - genetics; Plant Stems - growth & development; post‐harvest; Protein Kinases - genetics; Receptors; Regulator genes; Reverse transcriptase polymerase chain reaction; Rosa - enzymology; Rosa - genetics; Rosa hybrida; Senescence and abscission; Sequence Alignment; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Vegetative apparatus, growth and morphogenesis. Senescence
• ISSN: 0022-0957; 1460-2431
• DOI: 10.1093/jexbot/53.371.1223

The expression of two CTR‐gene homologues was investigated during flower senescence in two Rosa hybrida cultivars. A fragment of a gene for a protein kinase, termed RhCTR1 (GenBank Acc. No. AF271206), was amplified by PCR and used to isolate the corresponding full‐length cDNA (Acc. No. AY032953) from a rose petal cDNA library. The protein RhCTR1 has 66% amino acid identity to Arabidopsis CTR1. A fragment of a second CTR homologue, termed RhCTR2 (Acc. No. AY029067) is 69% identical to the corresponding region of RhCTR1. RhCTR1 expression increased during flower senescence, while RhCTR2 was constitutively expressed during flower development. The expression of both RhCTR1 and RhCTR2 was increased in response to exogenous ethylene.