Investigation of the Role of Ansocalcin in the Biomineralization in Goose Eggshell Matrix

The role of proteins in biomineralization and the mechanism of eggshell formation are not well understood. We have isolated and purified the major protein, ansocalcin from goose eggshell matrix. The amino acid sequence study indicates that ansocalcin is homologous to the chicken eggshell protein, ov...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2002-04, Vol.99 (8), p.5155-5159
Main Authors: Lakshminarayanan, Rajamani, Kini, R. Manjunatha, Valiyaveettil, Suresh
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Amino Acids - chemistry
Animals
Biomineralogy
Calcite
Calcium Carbonate - chemistry
Chemistry
Chromatography
Crystal morphology
Crystals
Egg Proteins - chemistry
Egg Proteins - metabolism
Egg Shell - chemistry
Eggshells
Geese
Lectins
Microscopy, Electron, Scanning
Minerals
Models, Chemical
Molecular Sequence Data
Nucleation
Physical Sciences
Protein Structure, Tertiary
Proteins
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Summary:The role of proteins in biomineralization and the mechanism of eggshell formation are not well understood. We have isolated and purified the major protein, ansocalcin from goose eggshell matrix. The amino acid sequence study indicates that ansocalcin is homologous to the chicken eggshell protein, ovocleidin 17, and C-type lectins. Ansocalcin nucleates polycrystalline aggregates of calcite crystals in in vitro mineralization experiments. The polycrystalline aggregates obtained at higher concentration of ansocalcin appears to be similar to the crystals observed at the mamillary layer of the eggshell.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.072658899