Interactions of temperature and angiotensin II in paraventricular neurons of rats in vitro

We recorded extracellular impulse activity of hypothalamic paraventricular neurons ( n=75) in rat brain slices during application of angiotensin II (ANG II, 10(-9)-10(-6) M) and/or temperature changes (32-42 degrees C). ANG II, with a threshold concentration of 10(-8) M, increased the firing rate in...

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Bibliographic Details
Published in:Pflügers Archiv 2002-05, Vol.444 (1-2), p.117-125
Main Authors: Dewald, Mathias, Braun, Hans A, Huber, Martin T, Zwingmann, Detlev, Roth, Joachim, Voigt, Karlheinz
Format: Article
Language:English
Subjects:
Angiotensin II
Angiotensin II - pharmacology
Animals
Body Temperature Regulation - physiology
Electric Stimulation
Electrophysiology
In Vitro Techniques
Male
Neurons
Neurons - drug effects
Neurons - physiology
Neurosecretion - drug effects
Paraventricular Hypothalamic Nucleus - cytology
Paraventricular Hypothalamic Nucleus - drug effects
Paraventricular Hypothalamic Nucleus - physiology
Patch-Clamp Techniques
Rats
Rats, Sprague-Dawley
Temperature
Water-Electrolyte Balance - physiology
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Summary:We recorded extracellular impulse activity of hypothalamic paraventricular neurons ( n=75) in rat brain slices during application of angiotensin II (ANG II, 10(-9)-10(-6) M) and/or temperature changes (32-42 degrees C). ANG II, with a threshold concentration of 10(-8) M, increased the firing rate in more than 80% of the neurons with strongest excitations occurring in bursting neurons. Increasing the temperature also raised the discharge rate in the majority of the neurons, often together with enhanced burst discharges. When ANG II was applied during ongoing sinusoidal temperature changes, its effects were more pronounced at elevated temperatures. These electrophysiological data illustrate that stimulus-encoding properties at the neuronal level can contribute to the interactions between osmoregulatory and thermoregulatory mechanisms including mutual sensitization when different stimuli (here: ANG II and temperature changes) are applied simultaneously.
ISSN:0031-6768
1432-2013
DOI:10.1007/s00424-002-0809-x