Production of a monoclonal antibody against Aeromonas hydrophila and its application to bacterial identification

• Published in: Journal of applied microbiology 2002-05, Vol.92 (5), p.936-940
• Main Authors: Delamare, A.P.L., Echeverrigaray, S., Duarte, K.R., Gomes, L.H., Costa, S.O.P.
• Format: Article
• Language: English
• Subjects: Aeromonas hydrophila - classification; Aeromonas hydrophila - immunology; Animals; Antibodies, Bacterial - biosynthesis; Antibodies, Bacterial - immunology; Antibodies, Monoclonal - biosynthesis; Antibodies, Monoclonal - immunology; Antibody Specificity; Bacteriological methods and techniques used in bacteriology; Bacteriology; Biological and medical sciences; Enzyme-Linked Immunosorbent Assay; Feces - microbiology; Fundamental and applied biological sciences. Psychology; Gram-Negative Bacterial Infections - diagnosis; Gram-Negative Bacterial Infections - microbiology; Humans; Hybridomas; Mice; Microbiology
• ISSN: 1364-5072; 1365-2672
• DOI: 10.1046/j.1365-2672.2002.01603.x

Aims: to develop a monoclonal antibody (MAb) for the rapid detection of Aeromonas hydrophila in human faeces. Methods and Results: A monoclonal antibody with strong specificity against Aer. hydrophila was obtained by the fusion of myeloma cells and splenocytes of a mouse immunized with vegetative cells of Aer. hydrophila ATCC 7966, followed by a two‐step selection against other species of the genera. ELISA analyses revealed that MAb 5F3 strongly reacts with all the Aer. hydrophila strains evaluated, showing a just basal reactivity against other species of the genera, especially Aer. sobria and Aer. caviae. Conclusions: MAb 5F3 was characterized as an IgG that recognized a polypeptide of approximately 110 kDa. Significance and Impact of the Study: This MAb could be used to detect Aer. hydrophila in human stool samples.