Dynamics of lipid accumulation by the fat body of Rhodnius prolixus: The involvement of lipophorin binding sites

• Published in: Journal of insect physiology 2008-05, Vol.54 (5), p.790-797
• Main Authors: Pontes, Emerson G., Leite, Paula, Majerowicz, David, Atella, Georgia C., Gondim, Katia C.
• Format: Article
• Language: English
• Subjects: Analysis of Variance; Animals; binding sites; Binding Sites - physiology; Fat body; Fat Body - metabolism; Fatty acid; Female; free fatty acids; hematophagy; lipids; lipophorin; Lipophorin receptor; lipophorin receptors; Lipoproteins - metabolism; Membranes - metabolism; nutrient reserves; nutrient transport; Palmitic Acid; radiolabeling; receptors; Rhodnius - metabolism; Rhodnius prolixus; temporal variation; Triacylglycerol; triacylglycerols; Triglycerides - metabolism; Triglycerides - pharmacokinetics; Tritium
• ISSN: 0022-1910; 1879-1611
• DOI: 10.1016/j.jinsphys.2008.02.003

In insects, lipids are stored in the fat body, mainly as triacylglycerol (TAG). In Rhodnius prolixus, a hematophagous hemipteran, lipids are accumulated after blood meal to be used later on. In adult females, at the second day after feeding, the amount of TAG was 57±17μg/fat body, it increased almost five times and at fourth day it was 244±35μg/fat body. TAG content remained constant until day 13, but it then decreased and, at day 20th it was very low (31±4.9μg/fat body). Radiolabeled free fatty acid was used to follow lipid accumulation by the fat body, as it was previously shown that, in R. prolixus, injected free fatty acids associate with lipophorin, a major hemolymphatic lipoprotein. 3H-palmitic acid was injected into the hemocoel of R. prolixus females. It disappeared from the hemolymph very rapidly, and radioactivity was incorporated by the fat body. Sixty minutes after injection, radioactivity in the fat body was found mainly in TAGs. The capacity of the fat body to incorporate fatty acids from the hemolymph varied according to the days after blood meal, and it was maximal around the fourth day. Lipophorin binding to specific sites in fat body membrane preparations also showed variation at different days. When membranes obtained from insects at the second, fifth and tenth days were compared, binding was highest at fifth day after feeding.