Serum tartrate-resistant acid phosphatase isoforms in rheumatoid arthritis

Objectives: Our objective was to evaluate the significance and source of serum tartrate-resistant acid phosphatase (TRACP) in patients with rheumatoid arthritis (RA). Methods: Thirty-five RA, 32 osteoarthritis (OA) and 16 control subjects were studied. Serum TRACP-5b activity and total TRACP protein...

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Bibliographic Details
Published in:Clinica chimica acta 2002-06, Vol.320 (1), p.49-58
Main Authors: Janckila, Anthony J., Neustadt, David H., Nakasato, Yuri R., Halleen, Jussi M., Hentunen, Teuvo, Yam, Lung T.
Format: Article
Language:English
Subjects:
Acid Phosphatase - blood
Adolescent
Adult
Aged
Aged, 80 and over
Arthritis, Rheumatoid - blood
Arthritis, Rheumatoid - enzymology
Biological and medical sciences
Biomarkers - blood
C-Reactive Protein - analysis
Cells, Cultured
Dendritic cell
Dendritic Cells - enzymology
Diseases of the osteoarticular system
Electrophoresis, Polyacrylamide Gel
Humans
Immunoassay
Inflammation
Inflammation - blood
Inflammation - enzymology
Inflammatory joint diseases
Isoenzymes - blood
Macrophage
Macrophages - enzymology
Medical sciences
Middle Aged
Osteoarthritis - blood
Osteoarthritis - enzymology
Rheumatoid arthritis
Sensitivity and Specificity
Tartrate-Resistant Acid Phosphatase
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Summary:Objectives: Our objective was to evaluate the significance and source of serum tartrate-resistant acid phosphatase (TRACP) in patients with rheumatoid arthritis (RA). Methods: Thirty-five RA, 32 osteoarthritis (OA) and 16 control subjects were studied. Serum TRACP-5b activity and total TRACP protein were determined by immunoassay. TRACP isoforms were analyzed by non-denaturing polyacrylamide gel electrophoresis (PAGE). Serum bone alkaline phosphatase (BAP), cross-linked N-terminal telopeptides (NTx), and C-terminal telopeptides (ICTP) of type I collagen were estimated as markers of bone turnover. C-reactive protein (CRP) was measured as a marker of chronic inflammation. Macrophages and dendritic cells (DC) were developed from peripheral blood monocytes. Cell lysates and culture supernatants were analyzed for TRACP isoforms by immunoassay and PAGE. Results: In RA, mean TRACP-5b activity was normal, but median total TRACP protein was increased twofold ( p
ISSN:0009-8981
1873-3492
DOI:10.1016/S0009-8981(02)00026-8