Effects of GH on lipid peroxidation and neutrophil superoxide anion-generating capacity in hypopituitary adults with GH deficiency

Summary objectives  Untreated growth hormone deficiency (GHD) is implicated in the increased cardiovascular risk associated with adult hypopituitarism. Oxidative stress, predisposing to lipid peroxidation, may be an important mediator of endothelial dysfunction, a pro‐atherogenic state associated wi...

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Published in:Clinical endocrinology (Oxford) 2002-04, Vol.56 (4), p.449-455
Main Authors: Smith, J. C., Lang, D., McEneny, J., Evans, L. M., Scanlon, M. F., Young, I., Davies, J.
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
Double-Blind Method
Female
Hormones. Endocrine system
Human Growth Hormone - deficiency
Human Growth Hormone - pharmacology
Human Growth Hormone - therapeutic use
Humans
Hypopituitarism - blood
Hypopituitarism - physiopathology
Lipid Peroxidation - drug effects
Lipoproteins, LDL - blood
Male
Medical sciences
Middle Aged
Neutrophils - drug effects
Neutrophils - metabolism
Oxidative Stress
Pharmacology. Drug treatments
Superoxides - blood
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Summary:Summary objectives  Untreated growth hormone deficiency (GHD) is implicated in the increased cardiovascular risk associated with adult hypopituitarism. Oxidative stress, predisposing to lipid peroxidation, may be an important mediator of endothelial dysfunction, a pro‐atherogenic state associated with adult GHD. design and patients In a randomized, double‐blind, placebo‐controlled study we investigated the effects of GH replacement on low‐density lipoprotein (LDL) oxidation and neutrophil superoxide (O2−) generating capacity in 32 GHD adults (19 males, 13 females; age range 19–64 years) over 3 months. Thirty age‐ and sex‐matched healthy controls were also studied. measurements Lipid hydroperoxides (HPOs) in plasma were measured using the ferrous oxidation with xylenol orange (FOX) assay. The susceptibility of LDL to oxidation was assessed by the copper‐catalysed lag phase of LDL oxidation. Neutrophil O2−generating capacity was assessed by a lucigenin‐based chemiluminescent assay of NADPH oxidase activity. Body composition was assessed using bioelectrical impedance analysis. results Compared to controls, GHD subjects had higher LDL cholesterol (4·0 ± 0·8 vs. 3·5 ± 0·9 mmol/l, P 
ISSN:0300-0664
1365-2265
DOI:10.1046/j.1365-2265.2002.01493.x