Notch Mediates the Segmental Specification of Angioblasts in Somites and Their Directed Migration toward the Dorsal Aorta in Avian Embryos

We studied, using avian embryos, mechanisms underlying the three-dimensional assembly of the dorsal aorta, the first-forming embryonic vessel in amniotes. This vessel originates from two distinct cell populations, the splanchnic and somitic mesoderms. We have unveiled a role for Notch signaling in t...

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Bibliographic Details
Published in:Developmental cell 2008-06, Vol.14 (6), p.890-901
Main Authors: Sato, Yuki, Watanabe, Tadayoshi, Saito, Daisuke, Takahashi, Teruaki, Yoshida, Shosei, Kohyama, Jun, Ohata, Emi, Okano, Hideyuki, Takahashi, Yoshiko
Format: Article
Language:English
Subjects:
Animals
Aorta - cytology
Aorta - embryology
Biological and medical sciences
Body Patterning - physiology
Cell differentiation, maturation, development, hematopoiesis
Cell Movement
Cell physiology
Chick Embryo
Coturnix
DEVBIO
DNA, Complementary
Electroporation
Embryo, Nonmammalian
Endothelium, Vascular
Fundamental and applied biological sciences. Psychology
Green Fluorescent Proteins - metabolism
Immunohistochemistry
Models, Biological
Molecular and cellular biology
Neovascularization, Physiologic
Receptor, Notch1 - metabolism
Receptors, Notch - genetics
Receptors, Notch - metabolism
RNA Interference
RNA, Messenger - metabolism
RNA, Small Interfering - metabolism
Signal Transduction
Somites - cytology
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Summary:We studied, using avian embryos, mechanisms underlying the three-dimensional assembly of the dorsal aorta, the first-forming embryonic vessel in amniotes. This vessel originates from two distinct cell populations, the splanchnic and somitic mesoderms. We have unveiled a role for Notch signaling in the somitic contribution. Upon activation of Notch signaling, a subpopulation of cells in the posterior half of individual somites migrates ventrally toward the primary dorsal aorta of splanchnic origin. After reaching the primary aorta, these somitic cells differentiate into the definitive aortic endothelial cells. This Notch-induced ventral migration is mediated by EphrinB2 and by an attractant action of the primary aorta. Furthermore, long-term chasing of cells by transposon-mediated gene transfer reveals that the segmentally provided endothelial cells of somitic origin in the dorsal aorta ultimately populate the entire region of the vessel. We demonstrate the molecular and cellular mechanisms underlying the formation of embryonic blood vessels from mesenchymal cells.
ISSN:1534-5807
1878-1551
DOI:10.1016/j.devcel.2008.03.024