Intra-abdominal Bacterial Infection Reactivates Latent Pulmonary Cytomegalovirus in Immunocompetent Mice

Critically ill surgery patients are susceptible to pulmonary reactivation of latent cytomegalovirus (CMV), but what triggers this reactivation is unknown. Immunosuppression and bacterial sepsis are thought to stimulate reactivation of CMV, and in this study it was hypothesized that immunosuppressive...

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Bibliographic Details
Published in:The Journal of infectious diseases 2002-05, Vol.185 (10), p.1395-1400
Main Authors: Cook, Charles H., Zhang, Yingxue, McGuinness, Brian J., Lahm, Michael C., Sedmak, Daniel D., Ferguson, Ronald M.
Format: Article
Language:English
Subjects:
Abdomen
Animals
Biological and medical sciences
Cecum - microbiology
Cecum - surgery
Critical care
Cytomegalovirus
Disease Models, Animal
DNA
Experimental mycoses and models
Female
Herpesviridae Infections - complications
Herpesviridae Infections - virology
Human viral diseases
Immunocompetence
Immunosuppression
Infections
Infectious diseases
Laparotomy
Lung - virology
Major Articles
Medical sciences
Mice
Mice, Inbred BALB C
Miscellaneous
Muromegalovirus - physiology
Mycoses
Pneumonia, Viral - complications
Pneumonia, Viral - virology
Polymerase chain reaction
Postoperative Complications
RNA
Sepsis
Sepsis - complications
Sepsis - microbiology
Viral diseases
Virus Activation
Virus Latency
Viruses
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Summary:Critically ill surgery patients are susceptible to pulmonary reactivation of latent cytomegalovirus (CMV), but what triggers this reactivation is unknown. Immunosuppression and bacterial sepsis are thought to stimulate reactivation of CMV, and in this study it was hypothesized that immunosuppressive effects of surgery with or without concomitant bacterial infection may reactivate latent CMV. Mice infected with CMV were allowed to develop latent infections. Latently infected mice underwent a laparotomy with cecal ligation and puncture (CLP; n = 30), a laparotomy alone (sham; n = 10), or no surgery (control; n = 5). Lung tissue homogenates were evaluated for viral activity, and, 2 and 3 weeks after CLP, lungs of 7 of 7 and 5 of 5 mice, respectively, showed reactivation of latent CMV. In contrast, lungs from all sham-operated animals and controls showed no viral reactivation. These findings demonstrate that surgery with subsequent intraabdominal bacterial infection reactivated CMV in lungs of latently infected mice. The mechanism of this reactivation is unknown but likely involves cytokines induced by sepsis.
ISSN:0022-1899
1537-6613
DOI:10.1086/340508