Use of 13C-labeled glucose for measuring exogenous glucose oxidation in mice

The author modified a respiratory gas analyzer to analyze the respiratory sup13 CO2 of 12 small laboratory animals all at once. To investigate the practical use of this system, mice were orally (OR) or intravenously (IV) given glucose solutions containing three different amounts of sup13 C-labeled g...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2002-02, Vol.66 (2), p.426-429
Main Authors: Ishihara, K. (Sugiyama Jogakuen Univ., Nagoya (Japan)), Oyaizu, S, Mizunoya, W, Fukuchi, Y, Yasumoto, K, Fushiki, T
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Carbohydrates
CARBON DIOXIDE
Carbon Isotopes
CELL RESPIRATION
Fundamental and applied biological sciences. Psychology
GLUCOSE
Glucose - metabolism
INJECTION
ISOTOPES
Male
MEASUREMENT
Metabolisms and neurohumoral controls
MICE
Mice, Inbred BALB C
ORAL ADMINISTRATION
OXIDATION
Oxidation-Reduction
TRACER TECHNIQUES
Vertebrates: anatomy and physiology, studies on body, several organs or systems
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Summary:The author modified a respiratory gas analyzer to analyze the respiratory sup13 CO2 of 12 small laboratory animals all at once. To investigate the practical use of this system, mice were orally (OR) or intravenously (IV) given glucose solutions containing three different amounts of sup13 C-labeled glucose. Expired sup13 CO2 derived from exogenous glucose was detected within 10 minutes after administration in OR mice, but about 30 minutes in IV mice. The height of the peak of sup13 CO2 expiration was correlated with the administered sup13 C-glucose mass.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.66.426