Effect of Cationic Liposomes in an in Vitro Transcription and Translation System

The effects of cationic liposomes complexed with plasmid DNA on the process of transcription was examined using a recently developed rapid cell free translation system. The findings indicate that the liposome itself inhibited the process when the ratio of DNA/liposome typically used in transfection...

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Bibliographic Details
Published in:Biological & pharmaceutical bulletin 2002, Vol.25(4), pp.529-531
Main Authors: Tachibana, Rieko, Harashima, Hideyoshi, Ishida, Tatsuhiro, Shinohara, Yasuo, Hino, Mami, Terada, Hiroshi, Baba, Yoshinobu, Kiwada, Hiroshi
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Biological and medical sciences
Biotechnology
Blotting, Western
cationic liposome
Cations
Fundamental and applied biological sciences. Psychology
gene delivery
General pharmacology
Genetic engineering
Genetic technics
Liposomes - pharmacology
Medical sciences
Methods. Procedures. Technologies
Molecular Sequence Data
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
Protein Biosynthesis - drug effects
Proteins - genetics
rapid translation system (RTS500)
Rats
transcription
Transcription, Genetic - drug effects
Vectors (cloning, transfer, expression). Insertion sequences and transposons
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Summary:The effects of cationic liposomes complexed with plasmid DNA on the process of transcription was examined using a recently developed rapid cell free translation system. The findings indicate that the liposome itself inhibited the process when the ratio of DNA/liposome typically used in transfection studies was used.
ISSN:0918-6158
1347-5215
DOI:10.1248/bpb.25.529