Identification of a Novel Ly49 Promoter That Is Active in Bone Marrow and Fetal Thymus

The analysis of several Ly49 genes has identified a tissue-specific promoter adjacent to the previously defined first exon. The current study reveals the presence of an additional Ly49 promoter (Pro-1) and two noncoding exons upstream of the previously defined promoter (Pro-2). DNA sequences homolog...

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Bibliographic Details
Published in:The Journal of immunology (1950) 2002-05, Vol.168 (10), p.5163-5169
Main Authors: Saleh, Ali, Makrigiannis, Andrew P, Hodge, Deborah L, Anderson, Stephen K
Format: Article
Language:English
Subjects:
5' Untranslated Regions - immunology
Alternative Splicing - immunology
Animals
Antigens, Ly - genetics
Base Sequence
Bone Marrow Cells - immunology
Bone Marrow Cells - metabolism
Cell Line
Exons - immunology
Fetus
Gene Expression Regulation - immunology
Killer Cells, Natural
Lectins, C-Type
Liver - cytology
Liver - immunology
Membrane Glycoproteins - genetics
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Molecular Sequence Data
Multigene Family - immunology
NK Cell Lectin-Like Receptor Subfamily A
Promoter Regions, Genetic - immunology
Receptors, NK Cell Lectin-Like
RNA, Messenger - isolation & purification
Thymus Gland - immunology
Thymus Gland - metabolism
Transcription Initiation Site
Transcription, Genetic - immunology
Tumor Cells, Cultured
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Summary:The analysis of several Ly49 genes has identified a tissue-specific promoter adjacent to the previously defined first exon. The current study reveals the presence of an additional Ly49 promoter (Pro-1) and two noncoding exons upstream of the previously defined promoter (Pro-2). DNA sequences homologous to Pro-1 are present 4-10 kb upstream of Pro-2 in all Ly49 genes examined, and Pro-1 transcripts were detected from the Ly49a, e, g, o, and v genes. Pro-1 activity can be detected in bone marrow, embryonic thymus, freshly isolated liver NK cells, and the murine LNK cell line, but it does not function in adult thymus, sorted NK-T cells, spleen NK cells, or the EL-4 T cell line, even though these cells express Ly49 proteins. Luciferase reporter assays identified a Pro-1 core promoter region that functions in the LNK cell line but not EL-4 cells. The novel promoter is not active in mature NK cells, suggesting that Pro-1 represents an early Ly49 promoter.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.168.10.5163