Drug Screening of Hair by Liquid Chromatography-Tandem Mass Spectrometry

Hair has become an important matrix for drug analysis, complementary to blood and urine as a matrix. A prolonged detection window makes hair analysis suitable for lhe detection of exposure to illegal and medicinal drugs for periods up to 12 months. In the present study, a liquid chromatography-tande...

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Bibliographic Details
Published in:Journal of analytical toxicology 2008-06, Vol.32 (5), p.364-372
Main Authors: Hegstad, S., Khiabani, H.Z., Kristoffersen, L., Kunøe, N., Lobmaier, P.P., Christophersen, A.S.
Format: Article
Language:English
Subjects:
Analysis
Biological and medical sciences
Chromatography, High Pressure Liquid
Drug addictions
General pharmacology
Hair - chemistry
Humans
Medical sciences
Pharmacology. Drug treatments
Reference Standards
Reproducibility of Results
Spectrometry, Mass, Electrospray Ionization
Street Drugs - analysis
Substance Abuse Detection - methods
Tandem Mass Spectrometry - methods
Toxicology
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Summary:Hair has become an important matrix for drug analysis, complementary to blood and urine as a matrix. A prolonged detection window makes hair analysis suitable for lhe detection of exposure to illegal and medicinal drugs for periods up to 12 months. In the present study, a liquid chromatography-tandem mass spectrometry (LC-MS-MS) method for drug screening in hair was developed and validated. To 20 mg of hair, 0.45 mL of acetonitrile/25 mM formic acid (5:95 v/v) and 50 µL of deuterated internal standards were added, and the sample was incubated in a water bath at 37°C for 18 h. LC separation was achieved with a Zorbax SB-Phenyl column (2.1 × 100 mm, 3.5-µm particle). Mass detection was performed by positive ion mode electrospray LC-MS-MS and included the following drugs/metabolites: nicotine, cotinine, morphine, 6-monoacetylmorphine, codeine, amphetamine, methamphetamine, 3,4-methylenedioxymethamphetamine, cocaine, benzoylecgonine, 7-aminonitrazepam, 7-aminoclonazepam, 7-aminoflunitrazepam, oxazepam, diazepam, alprazolam, zopiclone, zolpidem, carisoprodol, meprobamate, buprenorphine, and methadone. Within- and between-assay relative standard deviations varied from 2.0% to 12% and 2.7% to 15%, respectively. The accuracies were in the range of −24% to 16%, and recoveries ranged from 25% to 100%. The LC-MS-MS method proved to be simple and robust for the determination of drugs in hair. It has been used for authentic samples in our laboratory in the past year.
ISSN:0146-4760
1945-2403
DOI:10.1093/jat/32.5.364