Proteomic response to iron deficiency in tomato root

To know the root adjustment in response to iron deficiency, differentially displayed proteins in tomato roots of wild type and its iron uptake inefficient mutant T3238fer were analyzed by 2-DE and MALDI-TOF MS-based proteomic method under iron sufficiency and deficiency. Ninety-seven proteins were i...

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Bibliographic Details
Published in:Proteomics (Weinheim) 2008-06, Vol.8 (11), p.2299-2311
Main Authors: Li, Jie, Wu, Xu-Dong, Hao, Shan-Ting, Wang, Xiu-Jie, Ling, Hong-Qing
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Anemias. Hemoglobinopathies
Ascorbic Acid
Biochemistry - methods
Biological and medical sciences
Diseases of red blood cells
FER
Fundamental and applied biological sciences. Psychology
Gene Expression Regulation, Plant
Genes, Plant
Hematologic and hematopoietic diseases
Iron - deficiency
Iron - metabolism
Lycopersicon esculentum - metabolism
Medical sciences
Metabolic diseases
Metabolism pathway
Metals (hemochromatosis...)
Methionine - chemistry
Miscellaneous
Mutation
Other metabolic disorders
Oxidation-Reduction
Plant Proteins - chemistry
Plant Roots
Protein Folding
Proteins
Proteomics - methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Tomato
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Summary:To know the root adjustment in response to iron deficiency, differentially displayed proteins in tomato roots of wild type and its iron uptake inefficient mutant T3238fer were analyzed by 2-DE and MALDI-TOF MS-based proteomic method under iron sufficiency and deficiency. Ninety-seven proteins were identified, 63 of them were classified in various metabolic pathways. About 40 proteins involved in starch degradation, TCA and ascorbate cycles were upregulated under iron deficiency and grouped in a network together with glycolysis, whereas proteins for fructose metabolism were decreased. Proteins involved in methionine synthesis, cell wall synthesis, mitochondria ATP synthesis, vacuole ATPase, HSP70/90, etc. also revealed enhanced expression under iron deficiency, while proteins about redox homeostasis, transcription factors, kinases, etc. showed diversified changes. The responses are closely associated with energy metabolism, organic acid formation, root morphological change, redox and sulfur homeostasis, and signal transduction, which enhance iron uptake, reutilization and other adaptive changes. Most of the proteins affected by iron deficiency and fer mutation showed similar effect on individual proteins or pathways, but the independent function of FER to iron deficiency were statistically indicated.
ISSN:1615-9853
1615-9861
DOI:10.1002/pmic.200700942