Docosahexaenoic acid enhances segregation of lipids between : 2H-NMR study

Solid-state (2)H-NMR of [(2)H(31)]-N-palmitoylsphingomyelin ([(2)H(31)]16:0SM, PSM*), supplemented by differential scanning calorimetry, was used for the first time, to our knowledge, to investigate the molecular organization of the sphingolipid in 1:1:1 mol mixtures with 1-palmitoyl-2-oleoyl-sn-gly...

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Bibliographic Details
Published in:Biophysical journal 2008-07, Vol.95 (1), p.203-214
Main Authors: Soni, Smita P, LoCascio, Daniel S, Liu, Yidong, Williams, Justin A, Bittman, Robert, Stillwell, William, Wassall, Stephen R
Format: Article
Language:English
Subjects:
Complex Mixtures - chemistry
Computer Simulation
Docosahexaenoic Acids - chemistry
Hydrogen - chemistry
Lipid Bilayers - chemistry
Magnetic Resonance Spectroscopy
Models, Chemical
Models, Molecular
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Summary:Solid-state (2)H-NMR of [(2)H(31)]-N-palmitoylsphingomyelin ([(2)H(31)]16:0SM, PSM*), supplemented by differential scanning calorimetry, was used for the first time, to our knowledge, to investigate the molecular organization of the sphingolipid in 1:1:1 mol mixtures with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphoethanolamine (16:0-18:1PE, POPE) or 1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine (16:0-22:6PE, PDPE) and cholesterol. When compared with (2)H-NMR data for analogous mixtures of [(2)H(31)]16:0-18:1PE (POPE*) or [(2)H(31)]16:0-22:6PE (PDPE*) with egg SM and cholesterol, molecular interactions of oleic acid (OA) versus docosahexaenoic acid (DHA) are distinguished, and details of membrane architecture emerge. SM-rich, characterized by higher-order, and PE-rich, characterized by lower-order, domains
ISSN:1542-0086
DOI:10.1529/biophysj.107.123612