Chitin Hydrolysis by Listeria spp., Including L. monocytogenes

• Published in: Applied and Environmental Microbiology 2008-06, Vol.74 (12), p.3823-3830
• Main Authors: Leisner, J.J, Larsen, M.H, Jørgensen, R.L, Brøndsted, L, Thomsen, L.E, Ingmer, H
• Format: Article
• Language: English
• Subjects: Arthropoda; Bacterial proteins; Bacterial Proteins - genetics; Biological and medical sciences; Carbohydrates; Chitin - metabolism; Chitinases - genetics; Culture Media - chemistry; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Bacterial; Gene Expression Regulation, Enzymologic; Glucose - metabolism; Gram-positive bacteria; Hydrolysis; Listeria - metabolism; Listeria monocytogenes; Microbiology; Mollusca; Phylogeny; Physiology and Biotechnology; Polymers; Sequence Homology, Amino Acid; Studies; Temperature
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/AEM.02701-07

Listeria spp., including the food-borne pathogen Listeria monocytogenes, are ubiquitous microorganisms in the environment and thus are difficult to exclude from food processing plants. The factors that contribute to their multiplication and survival in nature are not well understood, but the ability to catabolize various carbohydrates is likely to be very important. One major source of carbon and nitrogen in nature is chitin, an insoluble linear β-1,4-linked polymer of N-acetylglucosamine (GlcNAc). Chitin is found in cell walls of fungi and certain algae, in the cuticles of arthropods, and in shells and radulae of molluscs. In the present study, we demonstrated that L. monocytogenes and other Listeria spp. are able to hydrolyze α-chitin. The chitinolytic activity is repressed by the presence of glucose in the medium, suggesting that chitinolytic activity is subjected to catabolite repression. Activity is also regulated by temperature and is higher at 30°C than at 37°C. In L. monocytogenes EGD, chitin hydrolysis depends on genes encoding two chitinases, lmo0105 (chiB) and lmo1883 (chiA), but not on a gene encoding a putative chitin binding protein (lmo2467). The chiB and chiA genes are phylogenetically related to various well-characterized chitinases. The potential biological implications of chitinolytic activity of Listeria are discussed.