Amino acid and fatty acid composition of follicular fluid as predictors of in-vitro embryo development

Abstract The value of using the amino acid and fatty acid composition of follicular fluid as predictors of embryo development was assessed in a bovine model of in-vitro maturation (IVM), IVF and blastocyst culture (IVC). A total of 445 cumulus–oocyte complexes (COC) aspirated from visually healthy f...

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Bibliographic Details
Published in:Reproductive biomedicine online 2008, Vol.16 (6), p.859-868
Main Authors: Sinclair, KD, Lunn, LA, Kwong, WY, Wonnacott, K, Linforth, RST, Craigon, J
Format: Article
Language:English
Subjects:
amino acids
Amino Acids - metabolism
Animals
blastocysts
Cattle
Cells, Cultured
Cumulus Cells - cytology
Embryo Culture Techniques
Embryonic Development
fatty acids
Fatty Acids - metabolism
Female
follicular fluid
Follicular Fluid - metabolism
IVF
Obstetrics and Gynecology
oocyte
Oocytes - cytology
Oocytes - growth & development
Principal Component Analysis
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Summary:Abstract The value of using the amino acid and fatty acid composition of follicular fluid as predictors of embryo development was assessed in a bovine model of in-vitro maturation (IVM), IVF and blastocyst culture (IVC). A total of 445 cumulus–oocyte complexes (COC) aspirated from visually healthy follicles underwent IVM and IVF singly ( n = 138) or in groups ( n = 307). Of these COC, 349 cleaved (78%) following IVF and 112 went on to form blastocysts (32% of cleaved) following IVC. Culture method (singly or in groups) had no effect on development. In contrast to fatty acids, which had no predictive value, the amino acid composition of follicular fluid was associated with morphological assessments of COC quality and with post-fertilization development to the blastocyst stage. Principal component analysis identified two amino acids (i.e. alanine and glycine) that had the highest value for predicting early post-fertilization development. The predictive value of these two amino acids, in terms of the percentage of oocytes that cleaved following IVF, was greatest for COC with the poorest morphological grades but, with respect to blastocyst yields, was independent of morphological grade, and so may serve as a useful additional non-invasive measure of COC quality.
ISSN:1472-6483
1472-6491
DOI:10.1016/S1472-6483(10)60153-8