Effects of probe contact pressure on in vivo optical spectroscopy

The short- and long-term effects of probe contact pressure on in vivo diffuse reflectance and fluorescence spectroscopy were investigated using an animal model. Elevation in probe contact pressure induced major profile alterations in the diffuse reflectance spectra between 400 and 650 nm, and led to...

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Bibliographic Details
Published in:Optics express 2008-03, Vol.16 (6), p.4250-4262
Main Authors: Ti, Yalin, Lin, Wei-Chiang
Format: Article
Language:English
Subjects:
Heart - physiology
Humans
Liver - physiology
Optics and Photonics
Photometry - methods
Pressure
Reproducibility of Results
Sensitivity and Specificity
Spectrometry, Fluorescence - methods
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Summary:The short- and long-term effects of probe contact pressure on in vivo diffuse reflectance and fluorescence spectroscopy were investigated using an animal model. Elevation in probe contact pressure induced major profile alterations in the diffuse reflectance spectra between 400 and 650 nm, and led to significant intensity increases in the fluorescence spectra. The pressure threshold that was required to induce statistically significant spectral alterations was dependent upon the type of tissue. The observed spectral alterations may be attributed to decreases in local blood volume, blood oxygenation, and tissue metabolism, resulting from high probe contact pressure.
ISSN:1094-4087
1094-4087
DOI:10.1364/OE.16.004250