Identification of Tissue-Specific MicroRNAs from Mouse

MicroRNAs (miRNAs) are a new class of noncoding RNAs, which are encoded as short inverted repeats in the genomes of invertebrates and vertebrates [1, 2]. It is believed that miRNAs are modulators of target mRNA translation and stability, although most target mRNAs remain to be identified. Here we de...

Full description

Saved in:
Bibliographic Details
Published in:Current biology 2002-04, Vol.12 (9), p.735-739
Main Authors: Lagos-Quintana, Mariana, Rauhut, Reinhard, Yalcin, Abdullah, Meyer, Jutta, Lendeckel, Winfried, Tuschl, Thomas
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Cloning, Molecular
Mice - genetics
MicroRNAs - genetics
MicroRNAs - metabolism
Molecular Sequence Data
Organ Specificity
Sequence Analysis, DNA
Tissue Distribution
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:MicroRNAs (miRNAs) are a new class of noncoding RNAs, which are encoded as short inverted repeats in the genomes of invertebrates and vertebrates [1, 2]. It is believed that miRNAs are modulators of target mRNA translation and stability, although most target mRNAs remain to be identified. Here we describe the identification of 34 novel miRNAs by tissue-specific cloning of approximately 21-nucleotide RNAs from mouse. Almost all identified miRNAs are conserved in the human genome and are also frequently found in nonmammalian vertebrate genomes, such as pufferfish. In heart, liver, or brain, it is found that a single, tissue-specifically expressed miRNA dominates the population of expressed miRNAs and suggests a role for these miRNAs in tissue specification or cell lineage decisions. Finally, a miRNA was identified that appears to be the fruitfly and mammalian ortholog of C. elegans lin-4 stRNA.
ISSN:0960-9822
1879-0445
DOI:10.1016/S0960-9822(02)00809-6