Detergent extract from chlamydial elementary bodies used as antigen for enzyme-linked immunosorbent assay

Soluble antigen (SA) from chlamydial elementary bodies (EBs) was extracted with N-lauroylsarcosine. The extracted SA composed of lipopolysaccharide (LPS) and proteins was compared with EBs using an enzyme-linked immunosorbent assay (ELISA). Patient sera from natural chlamydial infections exhibited E...

Full description

Saved in:
Bibliographic Details
Published in:Diagnostic microbiology and infectious disease 2002-04, Vol.42 (4), p.237-242
Main Authors: Quevedo Diaz, M.A, Melničáková, J, Kazár, J, Hoch, J, Senfleben, S, Lukáčová, M
Format: Article
Language:English
Subjects:
Adult
Antibodies, Bacterial - blood
Antigens, Bacterial - chemistry
Bacterial diseases
Bacteriological methods and techniques used in bacteriology
Bacteriology
Biological and medical sciences
Blotting, Western
Chlamydia
Chlamydia Infections - diagnosis
Chlamydia trachomatis - chemistry
Chlamydia trachomatis - immunology
Detergents - chemistry
Electrophoresis, Polyacrylamide Gel
Elementary bodies
ELISA
Enzyme-Linked Immunosorbent Assay - methods
Female
Fundamental and applied biological sciences. Psychology
Human bacterial diseases
Humans
Infectious diseases
Lipopolysaccharides - chemistry
Male
Medical sciences
Microbiology
Middle Aged
Miscellaneous
Sarcosine - analogs & derivatives
Sarcosine - chemistry
Soluble antigen
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Soluble antigen (SA) from chlamydial elementary bodies (EBs) was extracted with N-lauroylsarcosine. The extracted SA composed of lipopolysaccharide (LPS) and proteins was compared with EBs using an enzyme-linked immunosorbent assay (ELISA). Patient sera from natural chlamydial infections exhibited ELISA mean absorbance (A 492 and A 405/650) values 2–5 times higher with SA than with EBs, resulting in a better discrimination between positive and negative human sera.
ISSN:0732-8893
1879-0070
DOI:10.1016/S0732-8893(02)00362-0