Apg-2 has a chaperone-like activity similar to Hsp110 and is overexpressed in hepatocellular carcinomas

Hepatocellular carcinoma (HCC) is the fifth most common cancer in the world. We constructed subtracted cDNA libraries enriched with genes overexpressed in HCCs. Among the 17 genes identified were molecular chaperones, Hsp110, Hsp90B, and Hsp70-1. Expression of the Hsp110 family members was further a...

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Bibliographic Details
Published in:FEBS letters 2004-02, Vol.560 (1), p.19-24
Main Authors: Gotoh, Kazuhisa, Nonoguchi, Kohsuke, Higashitsuji, Hiroaki, Kaneko, Yoshiyuki, Sakurai, Toshiharu, Sumitomo, Yasuhiko, Itoh, Katsuhiko, Subjeck, John R, Fujita, Jun
Format: Article
Language:English
Subjects:
Animals
Apg-1
Apg-2
Apoptosis - genetics
Biological Assay
Carcinoma, Hepatocellular - genetics
Cercopithecus aethiops
Chaperone
Clone Cells
COS Cells
Gene Expression Regulation, Neoplastic
Green Fluorescent Proteins
HA, hemagglutinin
HCC, hepatocellular carcinoma
Heat-Shock Proteins - genetics
Heat-Shock Proteins - metabolism
Hepatocellular carcinoma
Hot Temperature
Hsp, heat shock protein
Hsp110
HSP110 Heat-Shock Proteins
Hsp70
HSP70 Heat-Shock Proteins - genetics
HSP70 Heat-Shock Proteins - metabolism
Humans
Ig, immunoglobulin
Immunohistochemistry
Liver Neoplasms - genetics
Luciferases - metabolism
Luminescent Proteins
Mice
Molecular Chaperones - metabolism
Mutation
NIH 3T3 Cells
PBS, phosphate-buffered saline
Protein Denaturation
Rb, retinoblastoma gene product
Recombinant Fusion Proteins - metabolism
Tumor Cells, Cultured
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Summary:Hepatocellular carcinoma (HCC) is the fifth most common cancer in the world. We constructed subtracted cDNA libraries enriched with genes overexpressed in HCCs. Among the 17 genes identified were molecular chaperones, Hsp110, Hsp90B, and Hsp70-1. Expression of the Hsp110 family members was further analyzed, and increased transcript levels of Hsp110 and Apg-2, but not Apg-1, were found in 12 and 14, respectively, of 18 HCCs. Immunohistochemical analysis demonstrated the overexpression of the proteins in tumor cells. Apg-2 had chaperone ability similar to Hsp110 in a thermal denaturation assay using luciferase, and showed anti-apoptotic activity. These results suggest that the Hsp110 family members play important roles in hepatocarcinogenesis through their chaperoning activities.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(04)00034-1