A simple, optimized method for the determination of sulphide in whole blood by GC–MS as a marker of bowel fermentation processes

Hydrogen sulphide is produced in human large intestine by anaerobic fermentation and may play a pathogenic role. An analytical method for determination of sulphide in whole blood using an extractive alkylation technique was optimised and validated for this purpose. The sample was mixed with organic...

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Bibliographic Details
Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2002-04, Vol.770 (1), p.255-259
Main Authors: Hyspler, Radomir, Ticha, Alena, Indrova, Monika, Zadak, Zdenek, Hysplerova, Lidmila, Gasparic, Jiri, Churacek, Jaroslav
Format: Article
Language:English
Subjects:
Alkylation
Biomarkers - blood
Catalysts
Fermentation
Gas chromatography
Gas Chromatography-Mass Spectrometry - methods
Hydrogen sulfide
Intestine, Large - physiology
Mass spectrometry
Reference Standards
Reproducibility of Results
Sensitivity and Specificity
Sulfides - blood
Sulphide
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Summary:Hydrogen sulphide is produced in human large intestine by anaerobic fermentation and may play a pathogenic role. An analytical method for determination of sulphide in whole blood using an extractive alkylation technique was optimised and validated for this purpose. The sample was mixed with organic phase containing pentafluorobenzyl bromide as an alkylating agent. The benzalkonium chloride was used as a phase-transfer catalyst. The quantitative determination was performed using GC–MS technique in selected ion monitoring mode. The blood levels of sulphide of healthy controls were measured (35–80 μ M/l). The method is versatile, reproducible (RSD=2.7%) and suitable for research of anaerobic fermentation in vivo.
ISSN:1570-0232
1873-376X
DOI:10.1016/S1570-0232(01)00632-8