Improved genetic transformation of the thermophilic cyanobacterium, Thermosynechococcus elongatus BP-1

We improved genetic transformation of the thermophilic cyanobacterium, Thermosynechococcus elongatus BP-1, by combining electroporation with a top agar method. Transformation was also improved when a disruptant of a putative type I restriction endonuclease (tll2230) was used as recipient cells. In p...

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Bibliographic Details
Published in:Plant and cell physiology 2004-02, Vol.45 (2), p.171-175
Main Authors: Iwai, M. (Tokyo Univ. (Japan)), Katoh, H, Katayama, M, Ikeuchi, M
Format: Article
Language:English
Subjects:
Abbreviation: CmR
Agar - pharmacology
BIOTECHNOLOGY
Cell Culture Techniques - methods
Cells, Cultured
chloramphenicol-resistant cassette
CYANOBACTERIA
Cyanobacteria - genetics
DNA - genetics
DNA - pharmacology
Electroporation - methods
Gene Expression Regulation, Bacterial - genetics
GENETIC TRANSFORMATION
Keywords: Cyanobacterium — Restriction endonuclease — Single-crossover recombination — Thermophilic — Thermosynechococcus elongatus — Transformation
Molecular Biology - methods
PHOTOSYSTEMS
Recombination, Genetic - genetics
Restriction Mapping - methods
Transformation, Genetic - genetics
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Summary:We improved genetic transformation of the thermophilic cyanobacterium, Thermosynechococcus elongatus BP-1, by combining electroporation with a top agar method. Transformation was also improved when a disruptant of a putative type I restriction endonuclease (tll2230) was used as recipient cells. In particular, some constructs, with which wild type has never been transformed, were successfully integrated into the tll2230-disruptant. Single-crossover recombination was detected more frequently than the double-crossover recombination. In accordance with the presence of all the homologs of pil genes in Synechocystis sp. PCC 6803, we found that T. elongatus is naturally transformable with exogenous DNA.
ISSN:0032-0781
1471-9053
DOI:10.1093/pcp/pch015