Molecular characterization, phylogeny, and expression of c-type and g-type lysozymes in brill ( Scophthalmus rhombus)

Lysozymes are key proteins of the innate immune system against bacterial infections. In this study we report the molecular cloning and characterization of the c-type and g-type lysozymes in brill ( Scophthalmus rhombus). Catalytic and other conserved residues required for functionality were identifi...

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Bibliographic Details
Published in:Fish & shellfish immunology 2008-07, Vol.25 (1), p.57-65
Main Authors: Jiménez-Cantizano, Rosa M., Infante, Carlos, Martin-Antonio, Beatriz, Ponce, Marian, Hachero, Ismael, Navas, Jose Ignacio, Manchado, Manuel
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Brill
c-Type
Digestion
Fish Diseases - enzymology
Fish Diseases - microbiology
Flatfish
Flatfishes - genetics
Flatfishes - metabolism
g-Type
Gene Expression Regulation, Enzymologic - drug effects
Gram-Negative Bacterial Infections - enzymology
Gram-Negative Bacterial Infections - veterinary
Innate immune system
Lipopolysaccharides - pharmacology
Lysozyme
Marine
Molecular Sequence Data
Muramidase - classification
Muramidase - genetics
Photobacterium - immunology
Photobacterium damselae
Phylogeny
Scophthalmus rhombus
Sequence Alignment
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Summary:Lysozymes are key proteins of the innate immune system against bacterial infections. In this study we report the molecular cloning and characterization of the c-type and g-type lysozymes in brill ( Scophthalmus rhombus). Catalytic and other conserved residues required for functionality were identified. Phylogenetic analysis revealed distinct evolutionary histories for each lysozyme type. Expression profiles of both lysozyme genes were studied in juvenile tissues using a real-time PCR approach. c-Type lysozyme was expressed mainly in stomach and liver, whereas the g-type was detected in all tissues with highest mRNA levels observed in the spleen. Induction experiments revealed that g-type transcripts increased significantly in head kidney after lipopolysaccharide (25- and 23-fold at 12 and 24 h, respectively) and Photobacterium damselae subsp. piscicida (17-fold at 24 h) treatments. In contrast, no induction was observed for c-type lysozyme. All these data suggest that g-type lysozyme is involved in the response against bacterial infections, whereas c-type lysozyme may also play a role in digestion.
ISSN:1050-4648
1095-9947
DOI:10.1016/j.fsi.2007.12.009