T type 1/type 2 subsets balance in B-cell chronic lymphocytic leukemia—the three-color flow cytometry analysis

• Published in: Leukemia research 2002-07, Vol.26 (7), p.657-660
• Main Authors: Podhorecka, Monika, Dmoszynska, Anna, Rolinski, Jacek, Wasik, Ewa
• Format: Article
• Language: English
• Subjects: B-CLL; Biological and medical sciences; Biomarkers; CD3 Complex - blood; CD4 Lymphocyte Count - instrumentation; CD4 Lymphocyte Count - methods; CD8 Antigens - blood; CD8-Positive T-Lymphocytes - chemistry; Disease Progression; Flow cytometry; Flow Cytometry - methods; Hematologic and hematopoietic diseases; Humans; IFNγ; IL-4; Immunity, Cellular; Interferon-gamma - blood; Interleukin-4 - blood; Leukemia, Lymphocytic, Chronic, B-Cell - immunology; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis; Medical sciences; T type 1/T type 2; Th1 Cells - chemistry; Th2 Cells - chemistry
• ISSN: 0145-2126; 1873-5835
• DOI: 10.1016/S0145-2126(01)00194-1

The impairments in immune cell functions as well as changes in cytokine network are the cause of malignant cell accumulation and secondary immune deficiencies in B-cell chronic lymphocytic leukemia (B-CLL). To broaden the knowledge of immune system in B-CLL we tried to evaluate the Th1/Th2 and Tc1/Tc2 balance in B-CLL patients in comparison with healthy individuals and changes of this pattern during disease progression. The three-color flow cytometry technique was used to analyze IL-4 and IFNγ expression in peripheral blood CD3+CD4+ and CD3+CD8+ cells. The results indicate the dominance of T type 1 cells and T-cell-mediated immunity in B-CLL patients that is however shifted towards T type 2 during disease progression.