Expression of bone morphogenetic protein-7 (BMP-7) in human prostate

BACKGROUND It is known that BMP is a factor that strongly induces bone formation in muscle tissue. However, the expression of BMP‐7 is not detected in normal bone tissues. Recently, we reported that the level of BMP‐7 expression was significantly higher in metastatic bone lesions than in normal bone...

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Published in:The Prostate 2004-04, Vol.59 (1), p.101-106
Main Authors: Masuda, Hiroshi, Fukabori, Yoshitatsu, Nakano, Katsuya, Shimizu, Nobuaki, Yamanaka, Hidetoshi
Format: Article
Language:English
Subjects:
Aged
androgen ablation
Biological and medical sciences
Bone Morphogenetic Protein 7
Bone Morphogenetic Proteins - biosynthesis
Bone Morphogenetic Proteins - genetics
Cell Line, Tumor
Dihydrotestosterone - metabolism
Disease Progression
Epithelial Cells
Gene Expression Regulation, Neoplastic
Gynecology. Andrology. Obstetrics
human prostate
Humans
Male
Medical sciences
Neoplasm Recurrence, Local
Neoplasms, Hormone-Dependent - genetics
Neoplasms, Hormone-Dependent - metabolism
Nephrology. Urinary tract diseases
polymerase chain reaction
prostate cancer
Prostatic Neoplasms - genetics
Prostatic Neoplasms - metabolism
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - biosynthesis
RNA, Messenger - genetics
Transforming Growth Factor beta
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Summary:BACKGROUND It is known that BMP is a factor that strongly induces bone formation in muscle tissue. However, the expression of BMP‐7 is not detected in normal bone tissues. Recently, we reported that the level of BMP‐7 expression was significantly higher in metastatic bone lesions than in normal bone and that the high expression of BMP‐7 in metastatic bone lesions was related to osteoblastic metastasis. The aim of this study was to investigate the level of BMP‐7 gene expression in normal prostate glandular tissues, newly diagnosed prostate cancer tissues, and local recurrent prostate cancer tissues. METHODS Total RNA was extracted from 23 samples of normal prostate glandular tissues, 28 samples of newly diagnosed prostate cancer tissues, and 23 samples of local recurrent prostate cancer tissues. We also examined the expression of BMP‐7 in human prostatic epithelial cells (HPECs) under conditions of androgen replacement. After reverse transcription of the RNA samples, the expression of BMP‐7 was measured by real‐time quantitative polymerase chain reaction (PCR). Glyceraldehyde phosphate dehydrogenase (GAPDH) was used as an internal standard. RESULTS The level of BMP‐7 expression was highest in the normal prostate glandular tissue (P 
ISSN:0270-4137
1097-0045
DOI:10.1002/pros.20030