ACYP1 Gene Possesses Two Alternative Splicing Forms That Induce Apoptosis

In human cell lines two products of the ACYP1 gene were detected by RT ‐ PCR. In addition to the expected amplicon corresponding to the CT form of acylphosphatase (320 bp) a second unexpected one (400 bp) was characterized as the result of an alternative splicing in which an extra 79 bp long exon is...

Full description

Saved in:
Bibliographic Details
Published in:IUBMB life 2004-01, Vol.56 (1), p.29-33
Main Authors: Degl'Innocenti, Donatella, Marzocchini, Riccardo, Malentacchi, Francesca, Ramazzotti, Matteo, Raugei, Giovanni, Ramponi, Giampietro
Format: Article
Language:English
Subjects:
Acid Anhydride Hydrolases - genetics
Acid Anhydride Hydrolases - physiology
Acylphosphatase
Alternative Splicing
Amino Acid Sequence
apoptosis
Apoptosis - genetics
Apoptosis - physiology
Base Sequence
DNA - genetics
Exons
gene splicing
HeLa Cells
Humans
Introns
Isoenzymes - genetics
Isoenzymes - physiology
Molecular Sequence Data
Recombinant Fusion Proteins - genetics
Recombinant Fusion Proteins - metabolism
Reverse Transcriptase Polymerase Chain Reaction
RT ‐ PCR
Transfection
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In human cell lines two products of the ACYP1 gene were detected by RT ‐ PCR. In addition to the expected amplicon corresponding to the CT form of acylphosphatase (320 bp) a second unexpected one (400 bp) was characterized as the result of an alternative splicing in which an extra 79 bp long exon is inserted between the two known exons. This new product, indicated as CTsv, was cloned and expressed. We performed the ectopic expression of the two alternative splicing forms. Both CT and CTsv products were able to induce a proapoptotic effect when expressed in HeLa cell line, despite the fact that the CTsv protein did not show any acylphosphatase activity. IUBMB Life, 56: 29‐33, 2004
ISSN:1521-6543
1521-6551
DOI:10.1080/15216540310001654349