Transformation of the medicinal basidiomycete Trametes versicolor to hygromycin B resistance by restriction enzyme mediated integration

Abstract Trametes versicolor, a white-rot basidiomycete, degrades cellulose and lignin as well as many recalcitrant chemicals. There have been many reports about the cloning of laccase and peroxidase genes of T. versicolor which are involved in lignin degradation. In order to analyze a gene function...

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Bibliographic Details
Published in:FEMS microbiology letters 2002-04, Vol.209 (2), p.273-276
Main Authors: Kim, Keunsook, Leem, Youngeun, Kim, Kyujoong, Kim, Kyunghoon, Choi, Hyoung T
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Basidiomycota - drug effects
Basidiomycota - enzymology
Basidiomycota - genetics
Biological and medical sciences
Cellulose
Cloning
DNA Restriction Enzymes - metabolism
Drug Resistance, Microbial - genetics
Enzymes
Fundamental and applied biological sciences. Psychology
Genes
Genetic transformation
Growth, nutrition, metabolism, transports, enzymes. Molecular biology
Hygromycin
Hygromycin B
Hygromycin B - pharmacology
Hygromycin B resistance
Integration
Laccase
Lignin
Microbiology
Mycological methods and techniques used in mycology
Mycology
Organic chemistry
Peroxidase
Plasmids
Restriction enzyme mediated integration
Rot
Trametes versicolor
Transformation, Genetic
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Summary:Abstract Trametes versicolor, a white-rot basidiomycete, degrades cellulose and lignin as well as many recalcitrant chemicals. There have been many reports about the cloning of laccase and peroxidase genes of T. versicolor which are involved in lignin degradation. In order to analyze a gene function and introduce foreign genes into an organism, genetic transformation is required. Here we have successfully transformed T. versicolor to hygromycin B resistance using pAN 7-1 plasmid by restriction enzyme mediated integration and have obtained many mutants in peroxidase activity and growing patterns. The transformation frequency was 25–50 transformants (μg plasmid DNA)−1. The transformants were quite stable after 10 consecutive transfers in non-selectable medium.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2002.tb11143.x