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Transformation of the medicinal basidiomycete Trametes versicolor to hygromycin B resistance by restriction enzyme mediated integration
Abstract Trametes versicolor, a white-rot basidiomycete, degrades cellulose and lignin as well as many recalcitrant chemicals. There have been many reports about the cloning of laccase and peroxidase genes of T. versicolor which are involved in lignin degradation. In order to analyze a gene function...
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| Published in: | FEMS microbiology letters 2002-04, Vol.209 (2), p.273-276 |
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| cites | cdi_FETCH-LOGICAL-c3523-a9fd06ca700ad2cda4e4eec3f4aa95454d011fe03fbad2c03eabde5ee5794a153 |
| container_end_page | 276 |
| container_issue | 2 |
| container_start_page | 273 |
| container_title | FEMS microbiology letters |
| container_volume | 209 |
| creator | Kim, Keunsook Leem, Youngeun Kim, Kyujoong Kim, Kyunghoon Choi, Hyoung T |
| description | Abstract
Trametes versicolor, a white-rot basidiomycete, degrades cellulose and lignin as well as many recalcitrant chemicals. There have been many reports about the cloning of laccase and peroxidase genes of T. versicolor which are involved in lignin degradation. In order to analyze a gene function and introduce foreign genes into an organism, genetic transformation is required. Here we have successfully transformed T. versicolor to hygromycin B resistance using pAN 7-1 plasmid by restriction enzyme mediated integration and have obtained many mutants in peroxidase activity and growing patterns. The transformation frequency was 25–50 transformants (μg plasmid DNA)−1. The transformants were quite stable after 10 consecutive transfers in non-selectable medium. |
| doi_str_mv | 10.1111/j.1574-6968.2002.tb11143.x |
| format | article |
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Trametes versicolor, a white-rot basidiomycete, degrades cellulose and lignin as well as many recalcitrant chemicals. There have been many reports about the cloning of laccase and peroxidase genes of T. versicolor which are involved in lignin degradation. In order to analyze a gene function and introduce foreign genes into an organism, genetic transformation is required. Here we have successfully transformed T. versicolor to hygromycin B resistance using pAN 7-1 plasmid by restriction enzyme mediated integration and have obtained many mutants in peroxidase activity and growing patterns. The transformation frequency was 25–50 transformants (μg plasmid DNA)−1. The transformants were quite stable after 10 consecutive transfers in non-selectable medium.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/j.1574-6968.2002.tb11143.x</identifier><identifier>PMID: 12007817</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Anti-Bacterial Agents - pharmacology ; Basidiomycota - drug effects ; Basidiomycota - enzymology ; Basidiomycota - genetics ; Biological and medical sciences ; Cellulose ; Cloning ; DNA Restriction Enzymes - metabolism ; Drug Resistance, Microbial - genetics ; Enzymes ; Fundamental and applied biological sciences. Psychology ; Genes ; Genetic transformation ; Growth, nutrition, metabolism, transports, enzymes. Molecular biology ; Hygromycin ; Hygromycin B ; Hygromycin B - pharmacology ; Hygromycin B resistance ; Integration ; Laccase ; Lignin ; Microbiology ; Mycological methods and techniques used in mycology ; Mycology ; Organic chemistry ; Peroxidase ; Plasmids ; Restriction enzyme mediated integration ; Rot ; Trametes versicolor ; Transformation, Genetic</subject><ispartof>FEMS microbiology letters, 2002-04, Vol.209 (2), p.273-276</ispartof><rights>2002 Federation of European Microbiological Societies 2002</rights><rights>2002 Federation of European Microbiological Societies</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3523-a9fd06ca700ad2cda4e4eec3f4aa95454d011fe03fbad2c03eabde5ee5794a153</citedby><cites>FETCH-LOGICAL-c3523-a9fd06ca700ad2cda4e4eec3f4aa95454d011fe03fbad2c03eabde5ee5794a153</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14275453$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/12007817$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Keunsook</creatorcontrib><creatorcontrib>Leem, Youngeun</creatorcontrib><creatorcontrib>Kim, Kyujoong</creatorcontrib><creatorcontrib>Kim, Kyunghoon</creatorcontrib><creatorcontrib>Choi, Hyoung T</creatorcontrib><title>Transformation of the medicinal basidiomycete Trametes versicolor to hygromycin B resistance by restriction enzyme mediated integration</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Abstract
Trametes versicolor, a white-rot basidiomycete, degrades cellulose and lignin as well as many recalcitrant chemicals. There have been many reports about the cloning of laccase and peroxidase genes of T. versicolor which are involved in lignin degradation. In order to analyze a gene function and introduce foreign genes into an organism, genetic transformation is required. Here we have successfully transformed T. versicolor to hygromycin B resistance using pAN 7-1 plasmid by restriction enzyme mediated integration and have obtained many mutants in peroxidase activity and growing patterns. The transformation frequency was 25–50 transformants (μg plasmid DNA)−1. The transformants were quite stable after 10 consecutive transfers in non-selectable medium.</description><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Basidiomycota - drug effects</subject><subject>Basidiomycota - enzymology</subject><subject>Basidiomycota - genetics</subject><subject>Biological and medical sciences</subject><subject>Cellulose</subject><subject>Cloning</subject><subject>DNA Restriction Enzymes - metabolism</subject><subject>Drug Resistance, Microbial - genetics</subject><subject>Enzymes</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Genetic transformation</subject><subject>Growth, nutrition, metabolism, transports, enzymes. Molecular biology</subject><subject>Hygromycin</subject><subject>Hygromycin B</subject><subject>Hygromycin B - pharmacology</subject><subject>Hygromycin B resistance</subject><subject>Integration</subject><subject>Laccase</subject><subject>Lignin</subject><subject>Microbiology</subject><subject>Mycological methods and techniques used in mycology</subject><subject>Mycology</subject><subject>Organic chemistry</subject><subject>Peroxidase</subject><subject>Plasmids</subject><subject>Restriction enzyme mediated integration</subject><subject>Rot</subject><subject>Trametes versicolor</subject><subject>Transformation, Genetic</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2002</creationdate><recordtype>article</recordtype><recordid>eNqVkdGK1TAQhoMo7nH1FSQoeteaNGnTeiHo4qpwxJv1OkzT6W4ObXNMWt36Ar626ba4IIqYmyGZb_6ZzE_IE85SHs-LQ8pzJZOiKso0YyxLxzo-S5Fe3yG7X6m7ZMeEKhPOKnVCHoRwYIzJjBX3yQmPZarkakd-XHgYQut8D6N1A3UtHa-Q9thYYwfoaA3BNtb1s8ERaaT7GAP9ij5Y4zrn6ejo1XzpF8QO9A31GGwYYTBI63m5jd6aG3Ecvs_9Kg4jNtQOI176m8YPyb0WuoCPtnhKPp-_vTh7n-w_vftw9nqfGJFnIoGqbVhhQDEGTWYakCgRjWglQJXLXDaM8xaZaOslzwRC3WCOmKtKAs_FKXm-6h69-zLF2XRvg8GugwHdFLTiRakqXv4TjAgrC8Ei-PQ38OAmH1cXdCY4KwpZZFWkXq6U8S4Ej60-etuDnzVnenFVH_RinV6s04urenNVX8fix1uLqY7Luy3dbIzAsw2AYKBro6fGhltOZiouR0Tu1cp9sx3O_zGCPv-4z9QikK8Cbjr-pTz50w9-Ai0p0uw</recordid><startdate>200204</startdate><enddate>200204</enddate><creator>Kim, Keunsook</creator><creator>Leem, Youngeun</creator><creator>Kim, Kyujoong</creator><creator>Kim, Kyunghoon</creator><creator>Choi, Hyoung T</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200204</creationdate><title>Transformation of the medicinal basidiomycete Trametes versicolor to hygromycin B resistance by restriction enzyme mediated integration</title><author>Kim, Keunsook ; Leem, Youngeun ; Kim, Kyujoong ; Kim, Kyunghoon ; Choi, Hyoung T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3523-a9fd06ca700ad2cda4e4eec3f4aa95454d011fe03fbad2c03eabde5ee5794a153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2002</creationdate><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Basidiomycota - drug effects</topic><topic>Basidiomycota - enzymology</topic><topic>Basidiomycota - genetics</topic><topic>Biological and medical sciences</topic><topic>Cellulose</topic><topic>Cloning</topic><topic>DNA Restriction Enzymes - metabolism</topic><topic>Drug Resistance, Microbial - genetics</topic><topic>Enzymes</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Genetic transformation</topic><topic>Growth, nutrition, metabolism, transports, enzymes. Molecular biology</topic><topic>Hygromycin</topic><topic>Hygromycin B</topic><topic>Hygromycin B - pharmacology</topic><topic>Hygromycin B resistance</topic><topic>Integration</topic><topic>Laccase</topic><topic>Lignin</topic><topic>Microbiology</topic><topic>Mycological methods and techniques used in mycology</topic><topic>Mycology</topic><topic>Organic chemistry</topic><topic>Peroxidase</topic><topic>Plasmids</topic><topic>Restriction enzyme mediated integration</topic><topic>Rot</topic><topic>Trametes versicolor</topic><topic>Transformation, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Keunsook</creatorcontrib><creatorcontrib>Leem, Youngeun</creatorcontrib><creatorcontrib>Kim, Kyujoong</creatorcontrib><creatorcontrib>Kim, Kyunghoon</creatorcontrib><creatorcontrib>Choi, Hyoung T</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medicine (ProQuest)</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection (Proquest) (PQ_SDU_P3)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>PML(ProQuest Medical Library)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>ProQuest Biological Science Journals</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Keunsook</au><au>Leem, Youngeun</au><au>Kim, Kyujoong</au><au>Kim, Kyunghoon</au><au>Choi, Hyoung T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transformation of the medicinal basidiomycete Trametes versicolor to hygromycin B resistance by restriction enzyme mediated integration</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2002-04</date><risdate>2002</risdate><volume>209</volume><issue>2</issue><spage>273</spage><epage>276</epage><pages>273-276</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Abstract
Trametes versicolor, a white-rot basidiomycete, degrades cellulose and lignin as well as many recalcitrant chemicals. There have been many reports about the cloning of laccase and peroxidase genes of T. versicolor which are involved in lignin degradation. In order to analyze a gene function and introduce foreign genes into an organism, genetic transformation is required. Here we have successfully transformed T. versicolor to hygromycin B resistance using pAN 7-1 plasmid by restriction enzyme mediated integration and have obtained many mutants in peroxidase activity and growing patterns. The transformation frequency was 25–50 transformants (μg plasmid DNA)−1. The transformants were quite stable after 10 consecutive transfers in non-selectable medium.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>12007817</pmid><doi>10.1111/j.1574-6968.2002.tb11143.x</doi><tpages>4</tpages></addata></record> |
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| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 2002-04, Vol.209 (2), p.273-276 |
| issn | 0378-1097 1574-6968 |
| language | eng |
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| source | Oxford Journals Online |
| subjects | Anti-Bacterial Agents - pharmacology Basidiomycota - drug effects Basidiomycota - enzymology Basidiomycota - genetics Biological and medical sciences Cellulose Cloning DNA Restriction Enzymes - metabolism Drug Resistance, Microbial - genetics Enzymes Fundamental and applied biological sciences. Psychology Genes Genetic transformation Growth, nutrition, metabolism, transports, enzymes. Molecular biology Hygromycin Hygromycin B Hygromycin B - pharmacology Hygromycin B resistance Integration Laccase Lignin Microbiology Mycological methods and techniques used in mycology Mycology Organic chemistry Peroxidase Plasmids Restriction enzyme mediated integration Rot Trametes versicolor Transformation, Genetic |
| title | Transformation of the medicinal basidiomycete Trametes versicolor to hygromycin B resistance by restriction enzyme mediated integration |
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