Detection of Human Immunoglobulin in Microchip and Conventional Capillary Electrophoresis with Contactless Conductivity Measurements

The detection of human immunoglobulin M (IgM) was performed using capacitively coupled contactless conductivity detection (CCD) in electrophoresis carried out in conventional capillaries as well as on glass and poly(meth-yl methacrylate) (PMMA) microdevices. Also achieved was the analyses of IgG (an...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2004-03, Vol.76 (5), p.1282-1288
Main Authors: Abad-Villar, Eva M, Tanyanyiwa, Jatisai, Fernández-Abedul, M. Teresa, Costa-García, Agustín, Hauser, Peter C
Format: Article
Language:English
Subjects:
Analytical chemistry
Chemistry
Chromatographic methods and physical methods associated with chromatography
Conductivity
Electric Conductivity
Electrochemical methods
Electrophoresis, Capillary - instrumentation
Electrophoresis, Capillary - methods
Enzyme-Linked Immunosorbent Assay
Exact sciences and technology
Experiments
Glass
Humans
Immunoassay
Immunoglobulin G - analysis
Immunoglobulin M - analysis
Immunoglobulins
Isoelectric Point
Miscellaneous
Other chromatographic methods
Polymethyl methacrylate
Proteins
Reproducibility of Results
Semiconductors
Sensitivity and Specificity
Time Factors
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Summary:The detection of human immunoglobulin M (IgM) was performed using capacitively coupled contactless conductivity detection (CCD) in electrophoresis carried out in conventional capillaries as well as on glass and poly(meth-yl methacrylate) (PMMA) microdevices. Also achieved was the analyses of IgG (an anti-human IgM) and the complex formed in the reaction between the two immunoreagents. It is demonstrated that CCD is a powerful tool suitable not only for the detection of antibodies but also for monitoring an immunological interaction. Conductivity measurements allow the direct determination of immunoreagents, and it is advantageous, since no labels are required. The immunoglobulin IgM has been taken as model analyte. The reproducibility of the analytical signal (RSD = 1%), sensitivity and limits of detection obtained for IgM (0.15 ng/mL in conventional capillaries and 34 ng/mL in microchips) are comparable to those previously obtained with amperometric detection. The immunological reaction was performed either in conventional microtiter plates as used in ELISA or in situ on the glass chip.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac0346656