The Q-Trap mass spectrometer, a novel tool in the study of protein glycosylation

The use of the recently introduced Q-Trap mass spectrometer in the study of protein glycosylation is described. The combined ion trap and triple quadrupole scan functions make it a powerful system in both oligosaccharide and glycopeptide analysis. Several oligosaccharides, both linear and branched,...

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Bibliographic Details
Published in:Journal of the American Society for Mass Spectrometry 2004-03, Vol.15 (3), p.413-423
Main Authors: Sandra, K, Devreese, B, Van Beeumen, J, Stals, I, Claeyssens, M
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Animals
Biological and medical sciences
Carbohydrates
Cattle
Fragments
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods
Glucans - chemistry
Glycopeptides
Glycopeptides - chemistry
Glycosylation
Ions
Mass spectrometry
Mass Spectrometry - instrumentation
Mass Spectrometry - methods
Miscellaneous
Molecular Structure
Oligosaccharides
Other biological molecules
Polysaccharides - analysis
Polysaccharides - chemistry
Proteins
Proteins - chemistry
Quadrupoles
Ribonucleases - chemistry
Spectral sensitivity
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Summary:The use of the recently introduced Q-Trap mass spectrometer in the study of protein glycosylation is described. The combined ion trap and triple quadrupole scan functions make it a powerful system in both oligosaccharide and glycopeptide analysis. Several oligosaccharides, both linear and branched, were analyzed to obtain information on sequence, linkage, and branching. Quadrupole like MS/MS spectra with ion trap sensitivity but without the typical ion trap low mass cut-off were obtained. To determine the origin of fragments and to reveal the existence of new ions, the MS 3 capabilities of the system proved to be useful. Glycopeptides were selectively detected in peptide mixtures using the triple quadrupole precursor ion scan function, either in off-line experiments or during LC/MS using information dependent acquisition (IDA).
ISSN:1044-0305
1879-1123
DOI:10.1016/j.jasms.2003.11.003