Connective tissue growth factor and its role in lung adenocarcinoma invasion and metastasis

Tumor invasion and metastasis cause most deaths in cancer patients. Connective tissue growth factor (CTGF), a secreted protein that binds to integrins, modulates the invasive behavior of certain human cancer cells, but few mechanistic details are known. We investigated the roles of CTGF and collapsi...

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Bibliographic Details
Published in:JNCI : Journal of the National Cancer Institute 2004-03, Vol.96 (5), p.364-375
Main Authors: CHANG, Cheng-Chi, SHIH, Jin-Yuan, JENG, Yung-Ming, SU, Jen-Liang, LIN, Been-Zen, CHEN, Szu-Ta, CHAU, Yat-Pang, YANG, Pan-Chyr, KUO, Min-Liang
Format: Article
Language:English
Subjects:
Adenocarcinoma - metabolism
Adenocarcinoma - prevention & control
Adenocarcinoma - secondary
Biological and medical sciences
Biomarkers, Tumor - metabolism
Cell Line, Tumor
Connective Tissue Growth Factor
Disease Progression
Gene Deletion
Gene Expression Regulation, Neoplastic
Humans
Immediate-Early Proteins - genetics
Immediate-Early Proteins - metabolism
Immunohistochemistry
Intercellular Signaling Peptides and Proteins - genetics
Intercellular Signaling Peptides and Proteins - metabolism
Lung cancer
Lung Neoplasms - metabolism
Lung Neoplasms - pathology
Lymphatic Metastasis
Medical research
Medical sciences
Mutation
Neoplasm Invasiveness
Nerve Tissue Proteins - metabolism
Oligonucleotides, Antisense
Phosphoproteins - metabolism
Plasmids
Pneumology
Proportional Hazards Models
Reverse Transcriptase Polymerase Chain Reaction
Survival Analysis
Transfection
Tumors of the respiratory system and mediastinum
Up-Regulation
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Summary:Tumor invasion and metastasis cause most deaths in cancer patients. Connective tissue growth factor (CTGF), a secreted protein that binds to integrins, modulates the invasive behavior of certain human cancer cells, but few mechanistic details are known. We investigated the roles of CTGF and collapsin response mediator protein 1 (CRMP-1) in metastasis and invasion of human lung adenocarcinoma. We compared vector control-transfected cells with corresponding CTGF gene-transfected cells. Invasive activity was measured with a modified Boyden chamber assay, and metastatic activity was measured in an animal model. We used CTGF deletion mutants, CTGF and CRMP-1 antisense oligonucleotides, and anti-integrin and anti-CRMP-1 antibodies to investigate the functional relationship between CTGF and CRMP-1. Expression of CTGF protein in 78 lung adenocarcinoma specimens was investigated immunohistochemically. All statistical tests were two-sided. Invasive (both P
ISSN:0027-8874
1460-2105
DOI:10.1093/jnci/djh059