Photothermal Monitoring of Redox State of Respiratory Chain in Single Live Cells

A method for monitoring respiratory chain (RC) activity in single live cells is described. It is based on the registration of the photothermal (PT) response after a laser pulse of a live single cell. The dependence of the PT-response amplitude and shape upon redox state of RC components was studied...

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Bibliographic Details
Published in:Photochemistry and photobiology 2002-05, Vol.75 (5), p.519-526
Main Authors: Lapotko, Dmitri, Romanovskaya, Tat'yana, Gordiyko, Elena
Format: Article
Language:English
Subjects:
Animals
Cytochrome
Cytochrome c Group - metabolism
Hepatocytes - metabolism
Hepatocytes - radiation effects
Lasers
Mice
Monitoring methods
Oxidation-Reduction
Oxygen Consumption - physiology
Oxygen Consumption - radiation effects
PHOTOSYNTHESIS AND BIO/CHEMILUMINESCENCE
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Summary:A method for monitoring respiratory chain (RC) activity in single live cells is described. It is based on the registration of the photothermal (PT) response after a laser pulse of a live single cell. The dependence of the PT-response amplitude and shape upon redox state of RC components was studied with a PT microscope for two in vitro models: (1) solutions of the RC component cytochrome c and (2) mice hepatocytes. The parameters of the PT responses differed for oxidized and reduced forms of cytochrome c solutions and for inhibited RC and intact RC. The latter difference may be caused by alteration of the quantum yields of thermal (nonradiative) relaxation for light-absorbing molecules, i.e. RC components, as they undergo reduction during RC inhibition.
ISSN:0031-8655
1751-1097
DOI:10.1562/0031-8655(2002)075<0519:PMORSO>2.0.CO;2